Ghrelin-like Peptide with Fatty Acid Modification and O-glycosylation in the Red Stingray, Dasyatis Akajei

Overview

Journal BMC Biochem

Publisher Biomed Central

Specialty Biochemistry

Date 2009 Dec 17

PMID 20003394

Citations 1

Authors

Hiroyuki Kaiya

Shiho Kodama

Koutaro Ishiguro

Kouhei Matsuda

Minoru Uchiyama

Mikiya Miyazato

Kenji Kangawa

Affiliations

Soon will be listed here.

Abstract

Background: Ghrelin (GRLN) is now known to be an appetite-stimulating and growth hormone (GH)-releasing peptide that is predominantly synthesized and secreted from the stomachs of various vertebrate species from fish to mammals. Here, we report a GRLN-like peptide (GRLN-LP) in a cartilaginous fish, the red stingray, Dasyatis akajei.

Results: The purified peptide contains 16 amino acids (GVSFHPQPRS10TSKPSA), and the serine residue at position 3 is modified by n-octanoic acid. The modification is the characteristic of GRLN. The six N-terminal amino acid residues (GVSFHP) were identical to another elasmobranch shark GRLN-LP that was recently identified although it had low identity with other GRLN peptides. Therefore, we designated this peptide stingray GRLN-LP. Uniquely, stingray GRLN-LP was O-glycosylated with mucin-type glycan chains [N-acetyl hexosamine (HexNAc)3 hexose(Hex)2] at threonine at position 11 (Thr-11) or both serine at position 10 (Ser-10) and Thr-11. Removal of the glycan structure by O-glycanase made the in vitro activity of stingray GRLN-LP decreased when it was evaluated by the increase in intracellular Ca2+ concentrations using a rat GHS-R1a-expressing cell line, suggesting that the glycan structure plays an important role for maintaining the activity of stingray GRLN-LP.

Conclusions: This study reveals the structural diversity of GRLN and GRLN-LP in vertebrates.

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PMID: 24863367 PMC: 7168572. DOI: 10.1002/mas.21411.

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