Identification of TRPM7 Channels in Human Intestinal Interstitial Cells of Cajal

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2009 Dec 10

PMID 19998500

Citations 16

Authors

Byung Joo Kim

Kyu Joo Park

Hyung Woo Kim

Seok Choi

Jae Yeoul Jun

In Youb Chang

Ju-Hong Jeon

Insuk So

Seon Jeong Kim

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract.

Methods: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs).

Results: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue.

Conclusion: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.

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