Protection Against Lethal Neospora Caninum Infection in Mice Induced by Heterologous Vaccination with a Mic1 Mic3 Knockout Toxoplasma Gondii Strain

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2009 Dec 10

PMID 19995895

Citations 5

Authors

Diana Marcela Penarete-Vargas

Marie Noelle Mevelec

Sarah Dion

Edouard Seche

Isabelle Dimier-Poisson

Thierry Fandeur

Affiliations

Soon will be listed here.

Abstract

Neospora caninum and Toxoplasma gondii are closely related, obligate intracellular parasites infecting a wide range of vertebrate hosts and causing abortion and neonatal morbidity and mortality. Several lines of evidence suggest that cross immunity between these two pathogens could be exploited in the design of strategies for heterologous vaccination. We assessed the ability of an attenuated strain of T. gondii ("mic1-3KO strain") conferring strong protection against chronic and congenital toxoplasmosis to protect mice against lethal N. caninum infection. Mice immunized with mic1-3KO tachyzoites by the oral and intraperitoneal routes developed a strong cellular Th1 response and displayed significant protection against lethal heterologous N. caninum infection, with survival rates of 70% and 80%, respectively, whereas only 30% of the nonimmunized mice survived. We report here the acquisition of heterologous protective immunity against N. caninum following immunization with a live attenuated mic1-3KO strain of T. gondii.

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