Interleukin-8 Induces Neutrophil Transendothelial Migration

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1991 Jan 1

PMID 1997402

Citations 71

Authors

W B Smith

J R Gamble

I Clark-Lewis

M A Vadas

Affiliations

Soon will be listed here.

Abstract

Interleukin-8 (IL-8) is a potent neutrophil chemotactic stimulant. We have used chemically synthesized IL-8 to investigate its role in human neutrophil adhesion and transendothelial migration. IL-8 enhanced the adhesiveness of human neutrophils to plastic, and to both unstimulated and tumour necrosis factor (TNF)-stimulated endothelial monolayers in vitro. Using a two-compartment model separated by a confluent endothelial monolayer, we have shown that IL-8 chemotactic stimulation induced transmigration across the monolayer of up to 87.4 +/- 2.1% of added neutrophils (compared to random unstimulated transmigration of 2.2 +/- 0.7%), while chemokinetic stimulation led to transmigration of 21 +/- 3.8% of neutrophils. Preincubation of endothelium with TNF also induced transmigration in this model, and was additive when combined with an IL-8 chemotactic stimulus. Endothelial permeability was increased at maximal rates of chemotactic transmigration, which may correlate with increased permeability of vessels at inflammatory sites in vivo. The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Citing Articles

Blockade of the Platelet-Driven CXCL7-CXCR1/2 Inflammatory Axis Prevents Murine Cerebral Aneurysm Formation and Rupture.

Nowicki K, Mittal A, Hudson J, DAngelo M, McDowell M, Cao C Transl Stroke Res. 2024; .

PMID: 39499487 DOI: 10.1007/s12975-024-01304-2.

Strategies to attenuate maladaptive inflammatory response associated with cardiopulmonary bypass.

Banerjee D, Feng J, Sellke F Front Surg. 2024; 11:1224068.

PMID: 39022594 PMC: 11251955. DOI: 10.3389/fsurg.2024.1224068.

Sided Stimulation of Endothelial Cells Modulates Neutrophil Trafficking in an In Vitro Sepsis Model.

Ahmad D, Linares I, Pietropaoli A, Waugh R, McGrath J Adv Healthc Mater. 2024; 13(21):e2304338.

PMID: 38547536 PMC: 11338706. DOI: 10.1002/adhm.202304338.

Osteogenic Differentiation of Human Periodontal Ligament Stromal Cells Influences Their Immunosuppressive Potential toward Allogenic CD4 T Cells.

Milek O, Tur D, Ahcin L, Voitseshyna O, Behm C, Andrukhov O Int J Mol Sci. 2023; 24(22).

PMID: 38003629 PMC: 10671619. DOI: 10.3390/ijms242216439.

Mitochondria as a therapeutic: a potential new frontier in driving the shift from tissue repair to regeneration.

Main E, Cruz T, Bowlin G Regen Biomater. 2023; 10:rbad070.

PMID: 37663015 PMC: 10468651. DOI: 10.1093/rb/rbad070.

References

Colditz I, Zwahlen R, DEWALD B, Baggiolini M . In vivo inflammatory activity of neutrophil-activating factor, a novel chemotactic peptide derived from human monocytes. Am J Pathol. 1989; 134(4):755-60. PMC: 1879797. View

Arnaout M . Structure and function of the leukocyte adhesion molecules CD11/CD18. Blood. 1990; 75(5):1037-50. View

Baggiolini M, Walz A, Kunkel S . Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989; 84(4):1045-9. PMC: 329758. DOI: 10.1172/JCI114265. View

Lawrence M, Smith C, Eskin S, McIntire L . Effect of venous shear stress on CD18-mediated neutrophil adhesion to cultured endothelium. Blood. 1990; 75(1):227-37. View

Furie M, McHugh D . Migration of neutrophils across endothelial monolayers is stimulated by treatment of the monolayers with interleukin-1 or tumor necrosis factor-alpha. J Immunol. 1989; 143(10):3309-17. View

Gamble J, Harlan J, Klebanoff S, Vadas M . Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor. Proc Natl Acad Sci U S A. 1985; 82(24):8667-71. PMC: 391497. DOI: 10.1073/pnas.82.24.8667. View

Langeler E, van Hinsbergh V . Characterization of an in vitro model to study the permeability of human arterial endothelial cell monolayers. Thromb Haemost. 1988; 60(2):240-6. View

Atkinson Y, Marasco W, Lopez A, Vadas M . Recombinant human tumor necrosis factor-alpha. Regulation of N-formylmethionylleucylphenylalanine receptor affinity and function on human neutrophils. J Clin Invest. 1988; 81(3):759-65. PMC: 442523. DOI: 10.1172/JCI113381. View

Furie M, Cramer E, Naprstek B, Silverstein S . Cultured endothelial cell monolayers that restrict the transendothelial passage of macromolecules and electrical current. J Cell Biol. 1984; 98(3):1033-41. PMC: 2113155. DOI: 10.1083/jcb.98.3.1033. View

10.

Foster S, Aked D, Schroder J, Christophers E . Acute inflammatory effects of a monocyte-derived neutrophil-activating peptide in rabbit skin. Immunology. 1989; 67(2):181-3. PMC: 1385254. View

11.

Lo S, Detmers P, Levin S, Wright S . Transient adhesion of neutrophils to endothelium. J Exp Med. 1989; 169(5):1779-93. PMC: 2189299. DOI: 10.1084/jem.169.5.1779. View

12.

Carveth H, Bohnsack J, McIntyre T, Baggiolini M, Prescott S, Zimmerman G . Neutrophil activating factor (NAF) induces polymorphonuclear leukocyte adherence to endothelial cells and to subendothelial matrix proteins. Biochem Biophys Res Commun. 1989; 162(1):387-93. DOI: 10.1016/0006-291x(89)92009-3. View

13.

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

14.

Gamble J, Vadas M . A new assay for the measurement of the attachment of neutrophils and other cell types to endothelial cells. J Immunol Methods. 1988; 109(2):175-84. DOI: 10.1016/0022-1759(88)90240-2. View

15.

Clark-Lewis I, Aebersold R, Ziltener H, Schrader J, HOOD L, Kent S . Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science. 1986; 231(4734):134-9. DOI: 10.1126/science.3079915. View

16.

Wall R, Harker L, Quadracci L, Striker G . Factors influencing endothelial cell proliferation in vitro. J Cell Physiol. 1978; 96(2):203-13. DOI: 10.1002/jcp.1040960209. View

17.

Strieter R, Kunkel S, Showell H, Remick D, Phan S, Ward P . Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-alpha, LPS, and IL-1 beta. Science. 1989; 243(4897):1467-9. DOI: 10.1126/science.2648570. View

18.

Besemer J, Hujber A, Kuhn B . Specific binding, internalization, and degradation of human neutrophil activating factor by human polymorphonuclear leukocytes. J Biol Chem. 1989; 264(29):17409-15. View

19.

Gimbrone Jr M, Obin M, Brock A, Luis E, Hass P, Hebert C . Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions. Science. 1989; 246(4937):1601-3. DOI: 10.1126/science.2688092. View

20.

Larsen C, Anderson A, Appella E, Oppenheim J, Matsushima K . The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science. 1989; 243(4897):1464-6. DOI: 10.1126/science.2648569. View