In Vitro Megakaryocyte Production and Platelet Biogenesis: State of the Art

Overview

Journal Transfus Med Rev

Specialty Hematology

Date 2009 Dec 8

PMID 19962573

Citations 39

Authors

Jo-Anna Reems

Nicolas Pineault

Sijie Sun

Affiliations

Soon will be listed here.

Abstract

The exciting and extraordinary capabilities of stem cells to proliferate and differentiate into numerous cell types not only offers promises for changing how diseases are treated but may also impact how transfusion medicine may be practiced in the future. The possibility of growing platelets in the laboratory to some day supplement and/or replace standard platelet products has clear advantages for blood centers and patients. Because of the high utilization of platelets by patients undergoing chemotherapy or receiving stem cell transplants, platelet transfusions have steadily increased over the past decades. This trend is likely to continue as the number of adult and pediatric patients receiving stem cell transplants is also continuously rising. As a result of increased demand, coupled with the short shelf-life of platelet concentrates, providing platelets to patients can stretch the resources of most blood centers and drive donor recruitment efforts, and on occasion, platelet shortages can compromise the care of thrombocytopenic patients.

Citing Articles

Developing a Multichannel Bioreactor with a Collagen Scaffold, ECM, and Cryoprecipitate to Significantly Produce Platelets from Umbilical Cord Blood Stem Cells.

Derakhty Gonbad M, Khoshfetrat A, Movassaghpour A, Sanaat Z, Nozad Charoudeh H Int J Hematol Oncol Stem Cell Res. 2023; 17(4):245-256.

PMID: 38076776 PMC: 10700094. DOI: 10.18502/ijhoscr.v17i4.13916.

Apoptosis in megakaryocytes: Safeguard and threat for thrombopoiesis.

Yang S, Wang L, Wu Y, Wu A, Huang F, Tang X Front Immunol. 2023; 13:1025945.

PMID: 36685543 PMC: 9845629. DOI: 10.3389/fimmu.2022.1025945.

Augmented Production of Platelets From Cord Blood With Euchromatic Histone Lysine Methyltransferase Inhibition.

Liu Y, Zhao J, Wang Y, Su P, Wang H, Liu C Stem Cells Transl Med. 2022; 11(9):946-958.

PMID: 35880582 PMC: 9492236. DOI: 10.1093/stcltm/szac048.

Isolation of megakaryocytes using magnetic cell separation and adverse effects induced by diclofenac toxicity in an experiment.

Bagmut I, Ivanov O, Sheremet M, Smirnov S, Kolisnyk I, Ivanova J J Med Life. 2022; 15(1):65-70.

PMID: 35186138 PMC: 8852649. DOI: 10.25122/jml-2020-0129.

Ricolinostat promotes the generation of megakaryocyte progenitors from human hematopoietic stem and progenitor cells.

Jiang J, Qin J, Li J, Lin X, Zhang B, Fan Z Stem Cell Res Ther. 2022; 13(1):54.

PMID: 35123563 PMC: 8817546. DOI: 10.1186/s13287-022-02722-5.

References

Mostafa S, Miller W, Papoutsakis E . Oxygen tension influences the differentiation, maturation and apoptosis of human megakaryocytes. Br J Haematol. 2000; 111(3):879-89. View

Kaushansky K . Thrombopoietin: the primary regulator of platelet production. Blood. 1995; 86(2):419-31. View

Ungerer M, Peluso M, Gillitzer A, Massberg S, Heinzmann U, Schulz C . Generation of functional culture-derived platelets from CD34+ progenitor cells to study transgenes in the platelet environment. Circ Res. 2004; 95(5):e36-44. DOI: 10.1161/01.RES.0000141700.96085.2e. View

Piacibello W, Sanavio F, Severino A, Garetto L, Dane A, Gammaitoni L . Ex vivo expansion of cord blood progenitors. Vox Sang. 1998; 74 Suppl 2:457-62. DOI: 10.1111/j.1423-0410.1998.tb05456.x. View

Su R, Li K, Yang M, Zhang X, Tsang K, Fok T . Platelet-derived growth factor enhances ex vivo expansion of megakaryocytic progenitors from human cord blood. Bone Marrow Transplant. 2001; 27(10):1075-80. DOI: 10.1038/sj.bmt.1703042. View

Scurfield G, RADLEY J . Aspects of platelet formation and release. Am J Hematol. 1981; 10(3):285-96. DOI: 10.1002/ajh.2830100308. View

Alexander W, Roberts A, Nicola N, Li R, Metcalf D . Deficiencies in progenitor cells of multiple hematopoietic lineages and defective megakaryocytopoiesis in mice lacking the thrombopoietic receptor c-Mpl. Blood. 1996; 87(6):2162-70. View

Bergmeier W, Burger P, Piffath C, Hoffmeister K, Hartwig J, Nieswandt B . Metalloproteinase inhibitors improve the recovery and hemostatic function of in vitro-aged or -injured mouse platelets. Blood. 2003; 102(12):4229-35. DOI: 10.1182/blood-2003-04-1305. View

Miyazaki R, Ogata H, Iguchi T, Sogo S, Kushida T, Ito T . Comparative analyses of megakaryocytes derived from cord blood and bone marrow. Br J Haematol. 2000; 108(3):602-9. DOI: 10.1046/j.1365-2141.2000.01854.x. View

10.

Kehrel B, Wierwille S, Clemetson K, Anders O, Steiner M, Knight C . Glycoprotein VI is a major collagen receptor for platelet activation: it recognizes the platelet-activating quaternary structure of collagen, whereas CD36, glycoprotein IIb/IIIa, and von Willebrand factor do not. Blood. 1998; 91(2):491-9. View

11.

Sullenbarger B, Bahng J, Gruner R, Kotov N, Lasky L . Prolonged continuous in vitro human platelet production using three-dimensional scaffolds. Exp Hematol. 2008; 37(1):101-10. PMC: 2676426. DOI: 10.1016/j.exphem.2008.09.009. View

12.

Tao H, Gaudry L, Rice A, Chong B . Cord blood is better than bone marrow for generating megakaryocytic progenitor cells. Exp Hematol. 1999; 27(2):293-301. DOI: 10.1016/s0301-472x(98)00050-2. View

13.

Norol F, Vitrat N, Cramer E, Guichard J, BURSTEIN S, Vainchenker W . Effects of cytokines on platelet production from blood and marrow CD34+ cells. Blood. 1998; 91(3):830-43. View

14.

Sun L, Tan P, Yap C, Hwang W, Koh L, Lim C . In vitro biological characteristics of human cord blood-derived megakaryocytes. Ann Acad Med Singap. 2004; 33(5):570-5. View

15.

Bornstein R, Garcia-Vela J, Gilsanz F, Auray C, Cales C . Cord blood megakaryocytes do not complete maturation, as indicated by impaired establishment of endomitosis and low expression of G1/S cyclins upon thrombopoietin-induced differentiation. Br J Haematol. 2001; 114(2):458-65. DOI: 10.1046/j.1365-2141.2001.02954.x. View

16.

Vitrat N, Pique C, Le Couedic J, Norol F, Larsen A, Katz A . Endomitosis of human megakaryocytes are due to abortive mitosis. Blood. 1998; 91(10):3711-23. View

17.

Debili N, Wendling F, Katz A, Guichard J, BRETON-GORIUS J, Hunt P . The Mpl-ligand or thrombopoietin or megakaryocyte growth and differentiative factor has both direct proliferative and differentiative activities on human megakaryocyte progenitors. Blood. 1995; 86(7):2516-25. View

18.

Gurney A, de Sauvage F, Moore M . Thrombocytopenia in c-mpl-deficient mice. Science. 1994; 265(5177):1445-7. DOI: 10.1126/science.8073287. View

19.

Sigurjonsson O, Gudmundsson K, Haraldsdottir V, Rafnar T, Agnarsson B, Gudmundsson S . Flt3/Flk-2 ligand in combination with thrombopoietin decreases apoptosis in megakaryocyte development. Stem Cells Dev. 2004; 13(2):183-91. DOI: 10.1089/154732804323046783. View

20.

Kaufman R, AIRO R, Pollack S, CROSBY W . Circulating megakaryocytes and platelet release in the lung. Blood. 1965; 26(6):720-31. View