Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2009 Dec 3

PMID 19951687

Citations 179

Authors

Krishanu Saha

Rudolf Jaenisch

Affiliations

Soon will be listed here.

Abstract

Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating "disease-specific" iPSCs. Here, we examine the challenges and assumptions in creating a disease model from a single cell of the patient. Both the kinetics of disease onset and progression as well as the spatial localization of disease in the patient's body are challenges to disease modeling. New tools in genetic modification, reprogramming, biomaterials, and animal models can be used for addressing these challenges.

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