The Effect of Gentian Violet on Virulent Properties of Candida Albicans

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2009 Nov 26

PMID 19937129

Citations 6

Authors

Su Ying

Sun Qing

Li Chunyang

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to evaluate the effect of gentian violet (GV) on phospholipase activity, proteinase activity and germ tube formation rate of Candida albicans. Both 12 phospholipase-positive and 12 proteinase-positive C. albicans isolates with Pz values <or=0.89 were obtained. A yeast suspension (1-3 x 10(7) cfu/ml) of each isolate was prepared. After a brief exposure (60 min) to sub-therapeutic concentrations (0.5 or 2 microg/ml) of GV, Pz value of phospholipase, Pz value of proteinase and germ tube formation rate were determined. Phospholipase activity, proteinase activity and germ tube formation rate in two groups exposed to GV were significantly lower than those in the group unexposed (P < 0.05). The results of this study indicated that sub-therapeutic concentrations of GV may lead to reduction in phospholipase activity, proteinase activity and germ tube formation, and then may suppress virulence and pathogenicity of C. albicans.

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References

Bahn Y, Sundstrom P . CAP1, an adenylate cyclase-associated protein gene, regulates bud-hypha transitions, filamentous growth, and cyclic AMP levels and is required for virulence of Candida albicans. J Bacteriol. 2001; 183(10):3211-23. PMC: 95223. DOI: 10.1128/JB.183.10.3211-3223.2001. View

Rocha C, Schroppel K, Harcus D, Marcil A, Dignard D, Taylor B . Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans. Mol Biol Cell. 2001; 12(11):3631-43. PMC: 60281. DOI: 10.1091/mbc.12.11.3631. View

Kadir T, Gumru B, Uygun-Can B . Phospholipase activity of Candida albicans isolates from patients with denture stomatitis: the influence of chlorhexidine gluconate on phospholipase production. Arch Oral Biol. 2007; 52(7):691-6. DOI: 10.1016/j.archoralbio.2006.12.008. View

Anil S, Samaranayake L . Brief exposure to antimycotics reduces the extracellular phospholipase activity of Candida albicans and Candida tropicalis. Chemotherapy. 2003; 49(5):243-7. DOI: 10.1159/000072448. View

Lyon J, de Resende M . Correlation between adhesion, enzyme production, and susceptibility to fluconazole in Candida albicans obtained from denture wearers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102(5):632-8. DOI: 10.1016/j.tripleo.2005.12.015. View

Banno Y, Yamada T, Nozawa Y . Secreted phospholipases of the dimorphic fungus, Candida albicans; separation of three enzymes and some biological properties. Sabouraudia. 1985; 23(1):47-54. DOI: 10.1080/00362178585380081. View

McDonald P, Craig W, Kunin C . Persistent effect of antibiotics on Staphylococcus aureus after exposure for limited periods of time. J Infect Dis. 1977; 135(2):217-23. DOI: 10.1093/infdis/135.2.217. View

Pizzo G, Giuliana G, Milici M, DAngelo M . Effect of antimicrobial mouthrinses on the in vitro adhesion of Candida albicans to human buccal epithelial cells. Clin Oral Investig. 2001; 5(3):172-6. DOI: 10.1007/s007840100121. View

Mayr A, Hinterberger G, Dierich M, Lass-Florl C . Interaction of serotonin with Candida albicans selectively attenuates fungal virulence in vitro. Int J Antimicrob Agents. 2005; 26(4):335-7. PMC: 2980867. DOI: 10.1016/j.ijantimicag.2005.07.006. View

10.

Ruhnke M, Morschhauser J . Development of simultaneous resistance to fluconazole in Candida albicans and Candida dubliniensis in a patient with AIDS. J Antimicrob Chemother. 2000; 46(2):291-5. DOI: 10.1093/jac/46.2.291. View

11.

Arai R, Sugita T, Nishikawa A . The anthracycline antitumor agents doxorubicin and daunorubicin reduce the activity of Candida albicans phospholipase B. Microbiol Immunol. 2004; 48(9):665-7. DOI: 10.1111/j.1348-0421.2004.tb03477.x. View

12.

Maccato M, Kaufman R . Fungal vulvovaginitis. Curr Opin Obstet Gynecol. 1991; 3(6):849-52. View

13.

Kadir T, Uygun B, Akyuz S . Prevalence of Candida species in Turkish children: relationship between dietary intake and carriage. Arch Oral Biol. 2004; 50(1):33-7. DOI: 10.1016/j.archoralbio.2004.07.004. View

14.

Price M, Wilkinson I, Gentry L . Plate method for detection of phospholipase activity in Candida albicans. Sabouraudia. 1982; 20(1):7-14. DOI: 10.1080/00362178285380031. View

15.

Mirbod F, Banno Y, Ghannoum M, Ibrahim A, Nakashima S, Kitajima Y . Purification and characterization of lysophospholipase-transacylase (h-LPTA) from a highly virulent strain of Candida albicans. Biochim Biophys Acta. 1995; 1257(2):181-8. DOI: 10.1016/0005-2760(95)00072-k. View

16.

Traboulsi R, Mukherjee P, Ghannoum M . In vitro activity of inexpensive topical alternatives against Candida spp. isolated from the oral cavity of HIV-infected patients. Int J Antimicrob Agents. 2008; 31(3):272-6. DOI: 10.1016/j.ijantimicag.2007.11.008. View

17.

Ghannoum M, Filler S, Ibrahim A, Fu Y, Edwards Jr J . Modulation of interactions of Candida albicans and endothelial cells by fluconazole and amphotericin B. Antimicrob Agents Chemother. 1992; 36(10):2239-44. PMC: 245483. DOI: 10.1128/AAC.36.10.2239. View

18.

Simon C, Tabrizi S . [On the in vitro sensitivity of Candida albicans to nystatin, trichomycin, amphotericin B and gentian violet]. Med Welt. 1967; 37:2164-8. View

19.

Sanglard D, Hube B, Monod M, Odds F, Gow N . A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence. Infect Immun. 1997; 65(9):3539-46. PMC: 175504. DOI: 10.1128/iai.65.9.3539-3546.1997. View

20.

Ombrella A, Racca L, Ramos L . [Protease and phospholipase activities of Candida albicans isolated from vaginal secretions with different pH values]. Rev Iberoam Micol. 2008; 25(1):12-6. DOI: 10.1016/s1130-1406(08)70004-4. View