Manure- and Biosolids-resident Murine Norovirus 1 Attachment to and Internalization by Romaine Lettuce

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2009 Nov 26

PMID 19933344

Citations 21

Authors

Jie Wei

Yan Jin

Tom Sims

Kalmia E Kniel

Affiliations

Soon will be listed here.

Abstract

The attachment of murine norovirus 1 (MNV) in biosolids, swine manure, and dairy manure to Romaine lettuce and internalization of this virus were evaluated. The MNV in animal manures had behavior similar to that of pure MNV; however, MNV in biosolids had significantly higher levels of attachment and internalization than pure MNV or MNV in manures. The incubation time did not affect the attachment of MNV in biosolids or manure. Confocal microscopy was used to observe MNV on lettuce after SYBR gold-labeled MNV was added directly to lettuce or after lettuce was submersed in labeled virus. MNV was observed on the lettuce surface, inside open cuts, and occasionally within stomata. In general, lettuce pieces with a long cut on the edge and short cuts on the stem was more likely to contain internalized MNV than intact lettuce pieces, as observed by confocal microscopy; however, while the difference was visible, it was not statistically significant. This study showed that the presence of MNV in biosolids may increase the risk of fresh produce contamination and that the MNV in open cuts and stomata is likely to be protected from sanitization.

Citing Articles

Nutritional, Energy and Sanitary Aspects of Swine Manure and Carcass Co-digestion.

Tapparo D, Rogovski P, Cadamuro R, Souza D, Bonatto C, Camargo A Front Bioeng Biotechnol. 2020; 8:333.

PMID: 32411682 PMC: 7200981. DOI: 10.3389/fbioe.2020.00333.

Fate of Foodborne Viruses in the "Farm to Fork" Chain of Fresh Produce.

Li D, De Keuckelaere A, Uyttendaele M Compr Rev Food Sci Food Saf. 2020; 14(6):755-770.

PMID: 32313514 PMC: 7162173. DOI: 10.1111/1541-4337.12163.

Presence and Persistence of in Water: The Impact on Microbial Quality of Water and Food Safety.

Liu H, Whitehouse C, Li B Front Public Health. 2018; 6:159.

PMID: 29900166 PMC: 5989457. DOI: 10.3389/fpubh.2018.00159.

Internalization and dissemination of human norovirus and Tulane virus in fresh produce is plant dependent.

Yang Z, Chambers H, DiCaprio E, Gao G, Li J Food Microbiol. 2017; 69:25-32.

PMID: 28941906 PMC: 6361382. DOI: 10.1016/j.fm.2017.07.015.

Bacterial Surface-Displayed GII.4 Human Norovirus Capsid Proteins Bound to HBGA-Like Molecules in Romaine Lettuce.

Wang M, Rong S, Tian P, Zhou Y, Guan S, Li Q Front Microbiol. 2017; 8:251.

PMID: 28265267 PMC: 5316531. DOI: 10.3389/fmicb.2017.00251.

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