High Resolution Cell Lineage Tracing Reveals Developmental Variability in Leech

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2009 Nov 20

PMID 19924812

Citations 11

Authors

Stephanie E Gline

Dian-Han Kuo

Alberto Stolfi

David A Weisblat

Affiliations

Soon will be listed here.

Abstract

Knowing the normal patterns of embryonic cell proliferation, migration, and differentiation is a cornerstone for understanding development. Yet for most species, the precision with which embryonic cell lineages can be determined is limited by technical considerations (the large numbers of cells, extended developmental times, opacity of the embryos), and these are exacerbated by the inherent variability of the lineages themselves. Here, we present an improved method of cell lineage tracing in the leech Helobdella, driving the expression of a nuclearly localized histone H2B:GFP (green fluorescent protein) fusion protein in selected lineages by microinjection of a plasmid vector. This construct generates a long lasting and minimally mosaic signal with single cell resolution, and does not disrupt the development of most lineages tested. We have validated this technique by elucidating details of cell lineages contributing to segmental and prostomial tissues that could not be observed with standard dextran lineage tracers.

Citing Articles

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