Specific Initiation by RNA Polymerase I in a Whole-cell Extract from Yeast

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Feb 1

PMID 1992452

Citations 46

Authors

M C SCHULTZ

S Y Choe

R H Reeder

Affiliations

Soon will be listed here.

Abstract

A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.

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