Ultrasmall Near-infrared Non-cadmium Quantum Dots for in Vivo Tumor Imaging

Overview

Journal Small

Date 2009 Nov 14

PMID 19911392

Citations 47

Authors

Jinhao Gao

Kai Chen

Renguo Xie

Jin Xie

Seulki Lee

Zhen Cheng

Xiaogang Peng

Xiaoyuan Chen

Affiliations

Soon will be listed here.

Abstract

The high tumor uptake of ultrasmall near-infrared quantum dots (QDs) attributed to the enhanced permeability and retention effect is reported. InAs/InP/ZnSe QDs coated by mercaptopropionic acid (MPA) exhibit an emission wavelength of about 800 nm (QD800-MPA) with very small hydrodynamic diameter (<10 nm). Using 22B and LS174T tumor xenograft models, in vivo and ex vivo imaging studies show that QD800-MPA is highly accumulated in the tumor area, which is very promising for tumor detection in living mice. The ex vivo elemental analysis (Indium) using inductively coupled plasma (ICP) spectrometry confirm the tumor uptake of QDs. The ICP data are consistent with the in vivo and ex vivo fluorescence imaging. Human serum albumin (HSA)-coated QD800-MPA nanoparticles (QD800-MPA-HSA) show reduced localization in mononuclear phagocytic system-related organs over QD800-MPA plausibly due to the low uptake of QD800-MPA-HSA in macrophage cells. QD800-MPA-HSA may have great potential for in vivo fluorescence imaging.

Citing Articles

Cu-chelated InP/ZnSe/ZnS QDs as PET/fluorescence dual-modal probe for tumor imaging.

Zhao Z, Otsuka A, Nakamura N, Tatsumi T, Nakatsui K, Tsuzukiishi T Sci Technol Adv Mater. 2025; 26(1):2463317.

PMID: 40065760 PMC: 11892056. DOI: 10.1080/14686996.2025.2463317.

Biological Interaction and Imaging of Ultrasmall Gold Nanoparticles.

Sang D, Luo X, Liu J Nanomicro Lett. 2023; 16(1):44.

PMID: 38047998 PMC: 10695915. DOI: 10.1007/s40820-023-01266-4.

Recent progress of emitting long-wavelength carbon dots and their merits for visualization tracking, target delivery and theranostics.

Li C, Huang J, Yuan L, Xie W, Ying Y, Li C Theranostics. 2023; 13(9):3064-3102.

PMID: 37284447 PMC: 10240821. DOI: 10.7150/thno.80579.

Microfluidic synthesis of quantum dots and their applications in bio-sensing and bio-imaging.

Cheng Y, Ling S, Geng Y, Wang Y, Xu J Nanoscale Adv. 2022; 3(8):2180-2195.

PMID: 36133767 PMC: 9417800. DOI: 10.1039/d0na00933d.

Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection.

Peerzade S, Makarova N, Sokolov I Nanomaterials (Basel). 2020; 10(5).

PMID: 32397124 PMC: 7279313. DOI: 10.3390/nano10050905.

References

Michalet X, Pinaud F, Bentolila L, Tsay J, Doose S, Li J . Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709):538-44. PMC: 1201471. DOI: 10.1126/science.1104274. View

Diagaradjane P, Orenstein-Cardona J, Colon-Casasnovas N, Deorukhkar A, Shentu S, Kuno N . Imaging epidermal growth factor receptor expression in vivo: pharmacokinetic and biodistribution characterization of a bioconjugated quantum dot nanoprobe. Clin Cancer Res. 2008; 14(3):731-41. DOI: 10.1158/1078-0432.CCR-07-1958. View

Cai W, Shin D, Chen K, Gheysens O, Cao Q, Wang S . Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects. Nano Lett. 2006; 6(4):669-76. DOI: 10.1021/nl052405t. View

Cai W, Hsu A, Li Z, Chen X . Are quantum dots ready for in vivo imaging in human subjects?. Nanoscale Res Lett. 2011; 2(6):265-281. PMC: 3050636. DOI: 10.1007/s11671-007-9061-9. View

Xie R, Chen K, Chen X, Peng X . InAs/InP/ZnSe Core/Shell/Shell Quantum Dots as Near-Infrared Emitters: Bright, Narrow-Band, Non-Cadmium Containing, and Biocompatible. Nano Res. 2010; 1(6):457-464. PMC: 2902876. DOI: 10.1007/s12274-008-8048-x. View

Liu W, Choi H, Zimmer J, Tanaka E, Frangioni J, Bawendi M . Compact cysteine-coated CdSe(ZnCdS) quantum dots for in vivo applications. J Am Chem Soc. 2007; 129(47):14530-1. PMC: 2587328. DOI: 10.1021/ja073790m. View

Law W, Yong K, Roy I, Ding H, Hu R, Zhao W . Aqueous-phase synthesis of highly luminescent CdTe/ZnTe core/shell quantum dots optimized for targeted bioimaging. Small. 2009; 5(11):1302-10. DOI: 10.1002/smll.200801555. View

Maeda H . The enhanced permeability and retention (EPR) effect in tumor vasculature: the key role of tumor-selective macromolecular drug targeting. Adv Enzyme Regul. 2001; 41:189-207. DOI: 10.1016/s0065-2571(00)00013-3. View

Tada H, Higuchi H, Wanatabe T, Ohuchi N . In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice. Cancer Res. 2007; 67(3):1138-44. DOI: 10.1158/0008-5472.CAN-06-1185. View

10.

Yong K, Ding H, Roy I, Law W, Bergey E, Maitra A . Imaging pancreatic cancer using bioconjugated InP quantum dots. ACS Nano. 2009; 3(3):502-10. PMC: 2762404. DOI: 10.1021/nn8008933. View

11.

Xiao Q, Huang S, Qi Z, Zhou B, He Z, Liu Y . Conformation, thermodynamics and stoichiometry of HSA adsorbed to colloidal CdSe/ZnS quantum dots. Biochim Biophys Acta. 2008; 1784(7-8):1020-7. DOI: 10.1016/j.bbapap.2008.03.018. View

12.

Desai N, Trieu V, Yao Z, Louie L, Ci S, Yang A . Increased antitumor activity, intratumor paclitaxel concentrations, and endothelial cell transport of cremophor-free, albumin-bound paclitaxel, ABI-007, compared with cremophor-based paclitaxel. Clin Cancer Res. 2006; 12(4):1317-24. DOI: 10.1158/1078-0432.CCR-05-1634. View

13.

Kim S, Zimmer J, Ohnishi S, Tracy J, Frangioni J, Bawendi M . Engineering InAs(x)P(1-x)/InP/ZnSe III-V alloyed core/shell quantum dots for the near-infrared. J Am Chem Soc. 2005; 127(30):10526-32. DOI: 10.1021/ja0434331. View

14.

Lim Y, Kim S, Nakayama A, Stott N, Bawendi M, Frangioni J . Selection of quantum dot wavelengths for biomedical assays and imaging. Mol Imaging. 2003; 2(1):50-64. DOI: 10.1162/15353500200302163. View

15.

Roco M . Nanotechnology: convergence with modern biology and medicine. Curr Opin Biotechnol. 2003; 14(3):337-46. DOI: 10.1016/s0958-1669(03)00068-5. View

16.

Stroh M, Zimmer J, Duda D, Levchenko T, Cohen K, Brown E . Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo. Nat Med. 2005; 11(6):678-82. PMC: 2686110. DOI: 10.1038/nm1247. View

17.

Katz E, Willner I . Integrated nanoparticle-biomolecule hybrid systems: synthesis, properties, and applications. Angew Chem Int Ed Engl. 2004; 43(45):6042-108. DOI: 10.1002/anie.200400651. View

18.

Ross P, Subramanian S . Thermodynamics of protein association reactions: forces contributing to stability. Biochemistry. 1981; 20(11):3096-102. DOI: 10.1021/bi00514a017. View

19.

Park J, Gu L, von Maltzahn G, Ruoslahti E, Bhatia S, Sailor M . Biodegradable luminescent porous silicon nanoparticles for in vivo applications. Nat Mater. 2009; 8(4):331-6. PMC: 3058936. DOI: 10.1038/nmat2398. View

20.

Cai W, Chen K, Li Z, Gambhir S, Chen X . Dual-function probe for PET and near-infrared fluorescence imaging of tumor vasculature. J Nucl Med. 2007; 48(11):1862-70. DOI: 10.2967/jnumed.107.043216. View