Monitoring the Dissemination of the Broad-host-range Plasmid PB10 in Sediment Microcosms by Quantitative PCR

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2009 Nov 10

PMID 19897757

Citations 7

Authors

Sebastien Bonot

Christophe Merlin

Affiliations

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Abstract

Studying the transfer of specific mobile genetic elements in complex environmental matrices remains difficult because suitable molecular tools are not yet available to back up classical culture-dependent approaches. In this report, we show that quantitative PCR could be used to monitor the dissemination of the broad-host-range plasmid pB10 in sediment microcosms. This approach lies in the differential measurement of the host and plasmid DNAs used to inoculate the microcosms, using a particular design of quantitative PCR primers/probes where we took advantage of the mosaic aspect of the bacterial genomes to achieve a highly specific quantitative PCR detection system.

Citing Articles

Experimental approaches to tracking mobile genetic elements in microbial communities.

Saak C, Dinh C, Dutton R FEMS Microbiol Rev. 2020; 44(5):606-630.

PMID: 32672812 PMC: 7476777. DOI: 10.1093/femsre/fuaa025.

Fate of Antibiotic Resistant and Broad Host Range Plasmid in Natural Soil Microcosms.

Fan X, Li H, Chen Q, Zhang Y, Ye J, Zhu Y Front Microbiol. 2019; 10:194.

PMID: 30881351 PMC: 6407330. DOI: 10.3389/fmicb.2019.00194.

Survival of Antibiotic Resistant Bacteria and Horizontal Gene Transfer Control Antibiotic Resistance Gene Content in Anaerobic Digesters.

Miller J, Novak J, Knocke W, Pruden A Front Microbiol. 2016; 7:263.

PMID: 27014196 PMC: 4781833. DOI: 10.3389/fmicb.2016.00263.

In Vivo and In Vitro Detection of Luminescent and Fluorescent Lactobacillus reuteri and Application of Red Fluorescent mCherry for Assessing Plasmid Persistence.

Karimi S, Ahl D, Vagesjo E, Holm L, Phillipson M, Jonsson H PLoS One. 2016; 11(3):e0151969.

PMID: 27002525 PMC: 4803345. DOI: 10.1371/journal.pone.0151969.

Natural microbial communities supporting the transfer of the IncP-1β plasmid pB10 exhibit a higher initial content of plasmids from the same incompatibility group.

Bellanger X, Guilloteau H, Breuil B, Merlin C Front Microbiol. 2014; 5:637.

PMID: 25505458 PMC: 4241820. DOI: 10.3389/fmicb.2014.00637.

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