A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2'-deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants

Overview

Journal Plant Cell Physiol

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2009 Nov 3

PMID 19880395

Citations 29

Authors

Yusuke Kakei

Isomaro Yamaguchi

Takanori Kobayashi

Michiko Takahashi

Hiromi Nakanishi

Takashi Yamakawa

Naoko K Nishizawa

Affiliations

Soon will be listed here.

Abstract

A highly sensitive quantitative method for assaying nicotianamine (NA) and 2'-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 mul of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.

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