Gene Expression During Successful Implantation in a Natural Cycle

Overview

Journal Fertil Steril

Specialty Reproductive Medicine

Date 2009 Nov 3

PMID 19880110

Citations 6

Authors

Inge Van Vaerenbergh

Ramsey McIntire

Leentje Van Lommel

Paul Devroey

Linda Giudice

Claire Bourgain

Affiliations

Soon will be listed here.

Abstract

Objective: To determine the human endometrial transcriptome during embryonic implantation.

Design: Case report.

Setting: Tertiary fertility center.

Patient(s): A 24-year-old woman who inadvertently became pregnant during an endometrial biopsy procedure.

Intervention(s): An endometrial biopsy was performed with a Pipelle device during the midluteal phase (days 19-21) of the cycle; blood samples for hormonal assessments were collected and a transvaginal ultrasound was performed.

Main Outcome Measure(s): Gene expression analysis of the endometrium during the window of implantation (during the implantation of an embryo) in a natural cycle. Localization of selected genes in endometrial tissue with immunohistochemistry.

Result(s): A total of 394 probe sets were differentially expressed in the pregnant sample when compared with the midsecretory phase nonpregnant endometrial samples. Different gene networks were involved, and selected genes from these signaling pathways were confirmed at the protein level.

Conclusion(s): Endometrial gene expression of a pregnant patient in a natural cycle is significantly different from nonpregnant patients during the midsecretory phase.

Citing Articles

Endometrial Receptivity Analysis (ERA): data versus opinions.

Ruiz-Alonso M, Valbuena D, Gomez C, Cuzzi J, Simon C Hum Reprod Open. 2021; 2021(2):hoab011.

PMID: 33880420 PMC: 8045472. DOI: 10.1093/hropen/hoab011.

15 years of transcriptomic analysis on endometrial receptivity: what have we learnt?.

Messaoudi S, El Kasmi I, Bourdiec A, Crespo K, Bissonnette L, Le Saint C Fertil Res Pract. 2019; 5:9.

PMID: 31396393 PMC: 6681490. DOI: 10.1186/s40738-019-0059-7.

Gene profiling the window of implantation: Microarray analyses from human and rodent models.

Herington J, Guo Y, Reese J, Paria B J Reprod Health Med. 2017; 2(Suppl 2):S19-S25.

PMID: 28239559 PMC: 5321051. DOI: 10.1016/j.jrhm.2016.11.006.

Progesterone/Estradiol Ratio as a Predictor in the ART Cycles with Premature Progesterone Elevation on the Day of hCG Trigger.

Mascarenhas M, Kamath M, Chandy A, Kunjummen A J Reprod Infertil. 2016; 16(3):155-61.

PMID: 26913234 PMC: 4508354.

Human Endometrial Transcriptomics: Implications for Embryonic Implantation.

Gomez E, Ruiz-Alonso M, Miravet J, Simon C Cold Spring Harb Perspect Med. 2015; 5(7):a022996.

PMID: 25818663 PMC: 4484960. DOI: 10.1101/cshperspect.a022996.

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