Direct Proof of a "more-than-single-layered" Peptidoglycan Architecture of Escherichia Coli W7: a Neutron Small-angle Scattering Study

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Jan 1

PMID 1987162

Citations 44

Authors

H Labischinski

E W Goodell

A Goodell

M L Hochberg

Affiliations

Soon will be listed here.

Abstract

A neutron small-angle scattering study was performed to determine the thickness and the scattering density profile of isolated peptidoglycan sacculi of Escherichia coli W7 in aqueous suspension (D2O). The maximum thickness (7 +/- 0.5 nm) of the sacculus from the exponential-phase cells was large enough to suggest the existence of a more-than-single-layered architecture. The experimental density profile across the thickness of the sacculus did not allow an unambiguous differentiation between a single-layered architecture characterized by completely extended peptide side chains projecting from the sugar strands or, alternatively, a partially triple layered structure. To resolve this ambiguity, sacculi were labeled with deuterated wall peptides. Comparison of the two experimental profiles indicated that the sacculus is more than single layered across its surface, with about 75 to 80% of its surface single layered and 20 to 25% triple layered.

Citing Articles

Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in antibiotic responses.

Skalnik C, Cheah S, Yang M, Wolff M, Spangler R, Talman L PLoS Comput Biol. 2023; 19(6):e1011232.

PMID: 37327241 PMC: 10310025. DOI: 10.1371/journal.pcbi.1011232.

An Updated Model of the Divisome: Regulation of the Septal Peptidoglycan Synthesis Machinery by the Divisome.

Attaibi M, den Blaauwen T Int J Mol Sci. 2022; 23(7).

PMID: 35408901 PMC: 8998562. DOI: 10.3390/ijms23073537.

Evolution of the analytical scattering model of live .

Semeraro E, Marx L, Mandl J, Frewein M, Scott H, Prevost S J Appl Crystallogr. 2021; 54(Pt 2):473-485.

PMID: 33953653 PMC: 8056759. DOI: 10.1107/S1600576721000169.

Peptidoglycan: Structure, Synthesis, and Regulation.

Garde S, Chodisetti P, Reddy M EcoSal Plus. 2021; 9(2).

PMID: 33470191 PMC: 11168573. DOI: 10.1128/ecosalplus.ESP-0010-2020.

Minimal exposure of lipid II cycle intermediates triggers cell wall antibiotic resistance.

Piepenbreier H, Diehl A, Fritz G Nat Commun. 2019; 10(1):2733.

PMID: 31227716 PMC: 6588590. DOI: 10.1038/s41467-019-10673-4.

References

Murray R, STEED P, ELSON H . THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965; 11:547-60. DOI: 10.1139/m65-072. View

Botta G, Park J . Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation. J Bacteriol. 1981; 145(1):333-40. PMC: 217277. DOI: 10.1128/jb.145.1.333-340.1981. View

Torbet J, Norton M . The structure of the cell wall of Staphylococcus aureus studied with neutron scattering and magnetic birefringence. FEBS Lett. 1982; 147(2):201-6. DOI: 10.1016/0014-5793(82)81042-9. View

Beveridge T, Davies J . Cellular responses of Bacillus subtilis and Escherichia coli to the Gram stain. J Bacteriol. 1983; 156(2):846-58. PMC: 217903. DOI: 10.1128/jb.156.2.846-858.1983. View

Burman L, Park J . Molecular model for elongation of the murein sacculus of Escherichia coli. Proc Natl Acad Sci U S A. 1984; 81(6):1844-8. PMC: 345018. DOI: 10.1073/pnas.81.6.1844. View

Hobot J, Carlemalm E, Villiger W, Kellenberger E . Periplasmic gel: new concept resulting from the reinvestigation of bacterial cell envelope ultrastructure by new methods. J Bacteriol. 1984; 160(1):143-52. PMC: 214693. DOI: 10.1128/jb.160.1.143-152.1984. View

Koch A, Burdett I . The variable T model for gram-negative morphology. J Gen Microbiol. 1984; 130(9):2325-38. DOI: 10.1099/00221287-130-9-2325. View

Labischinski H, Barnickel G, Naumann D, Keller P . Conformational and topological aspects of the three-dimensional architecture of bacterial peptidoglycan. Ann Inst Pasteur Microbiol (1985). 1985; 136A(1):45-50. DOI: 10.1016/s0769-2609(85)80020-x. View

Goodell E . Recycling of murein by Escherichia coli. J Bacteriol. 1985; 163(1):305-10. PMC: 219113. DOI: 10.1128/jb.163.1.305-310.1985. View

10.

Begg K, DONACHIE W . Cell shape and division in Escherichia coli: experiments with shape and division mutants. J Bacteriol. 1985; 163(2):615-22. PMC: 219166. DOI: 10.1128/jb.163.2.615-622.1985. View

11.

Goodell E, Higgins C . Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. J Bacteriol. 1987; 169(8):3861-5. PMC: 212484. DOI: 10.1128/jb.169.8.3861-3865.1987. View

12.

Glauner B, Holtje J, Schwarz U . The composition of the murein of Escherichia coli. J Biol Chem. 1988; 263(21):10088-95. View

13.

de Jonge B, Wientjes F, Jurida I, Driehuis F, Wouters J, Nanninga N . Peptidoglycan synthesis during the cell cycle of Escherichia coli: composition and mode of insertion. J Bacteriol. 1989; 171(11):5783-94. PMC: 210437. DOI: 10.1128/jb.171.11.5783-5794.1989. View

14.

Leduc M, Frehel C, Siegel E, van Heijenoort J . Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli. J Gen Microbiol. 1989; 135(5):1243-54. DOI: 10.1099/00221287-135-5-1243. View

15.

de Petris S . Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers. J Ultrastruct Res. 1967; 19(1):45-83. DOI: 10.1016/s0022-5320(67)80059-5. View

16.

Helmstetter C . DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968; 31(3):507-18. DOI: 10.1016/0022-2836(68)90424-5. View

17.

Schwarz U, Asmus A, Frank H . Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969; 41(3):419-29. DOI: 10.1016/0022-2836(69)90285-x. View

18.

Braun V, Gnirke H, Henning U, Rehn K . Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope. J Bacteriol. 1973; 114(3):1264-70. PMC: 285390. DOI: 10.1128/jb.114.3.1264-1270.1973. View

19.

Spratt B . Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc Natl Acad Sci U S A. 1975; 72(8):2999-3003. PMC: 432906. DOI: 10.1073/pnas.72.8.2999. View

20.

Burge R, Fowler A, Reaveley D . Structure of the peptidogylcan of bacterial cell walls. I. J Mol Biol. 1977; 117(4):927-53. DOI: 10.1016/s0022-2836(77)80006-5. View