ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H-Thymidine-Labeled Maize Nuclei

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2009 Oct 30

PMID 19866619

Citations 4

Authors

J K Koehler

K Muhlethaler

A Freywyssling

Affiliations

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Abstract

An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electron microscopically by this technique and were found to display nuclear labeling with impressive clarity. The new procedure utilized here yields objects in which the finest cell structures are easily resolvable without recourse to gelatin digestion techniques.

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