Application of Tissue-engineered Cartilage with BMP-7 Gene to Repair Knee Joint Cartilage Injury in Rabbits

Overview

Journal Knee Surg Sports Traumatol Arthrosc

Publisher Wiley

Specialties Emergency Medicine
General Surgery
Orthopedics

Date 2009 Oct 27

PMID 19855958

Citations 11

Authors

J H Che

Z R Zhang

G Z Li

W H Tan

X D Bai

F J Qu

Affiliations

Soon will be listed here.

Abstract

Injured articular cartilage has a poor capacity for spontaneous healing. So far, satisfactory solution to this subsistent problem has not been found, but transgenic therapy may be a promising way. This study aims to evaluate the effectiveness of a tissue-engineered cartilage that was transfected with morphogenetic protein 7 (BMP 7) in repairing the cartilaginous defects of rabbit knee joints. Chondrocytes were transfected with BMP-7 gene (5 x 10(6) cells/ml), inoculated into the collagen-fibrin gel scaffolds, and cultured for 14 days. Then, the scaffolds were implanted onto the created defects (5.0 mm in diameter) in rabbits' knee joints. After 12 weeks, the rabbits were sacrificed and histological sections were evaluated using modified O'Driscoll cartilage scores; In situ hybridization and immunohistochemistry were performed to detect the expression of BMP-7 mRNA and BMP-7 at the implanted site while the content of DNA and GAG was determined as well. A better quality of repairs was observed at the 12th week after implantation when compared to the control group using histological analyses. The content of DNA and specific secretion of GAG in the treatment group is statistically significant different compared with the control group. Gene therapy may be a promising treatment method, but the novel therapy approach needs further studies with respect to a longer follow-up period.

Citing Articles

Biomaterial-guided delivery of gene vectors for targeted articular cartilage repair.

Cucchiarini M, Madry H Nat Rev Rheumatol. 2018; 15(1):18-29.

PMID: 30514957 DOI: 10.1038/s41584-018-0125-2.

Cotransfected human chondrocytes: over-expression of IGF-I and SOX9 enhances the synthesis of cartilage matrix components collagen-II and glycosaminoglycans.

Simental-Mendia M, Lara-Arias J, Alvarez-Lozano E, Said-Fernandez S, Soto-Dominguez A, Padilla-Rivas G Braz J Med Biol Res. 2015; 48(12):1063-70.

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Gene Therapy for Cartilage Repair.

Madry H, Orth P, Cucchiarini M Cartilage. 2015; 2(3):201-25.

PMID: 26069580 PMC: 4300805. DOI: 10.1177/1947603510392914.

Tissue-engineering strategies to repair joint tissue in osteoarthritis: nonviral gene-transfer approaches.

Madry H, Cucchiarini M Curr Rheumatol Rep. 2014; 16(10):450.

PMID: 25182678 DOI: 10.1007/s11926-014-0450-7.

Using genes to facilitate the endogenous repair and regeneration of orthopaedic tissues.

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