Effect of Glycosylation and Additional Domains on the Thermostability of a Family 10 Xylanase Produced by Thermopolyspora Flexuosa

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2009 Oct 27

PMID 19854928

Citations 12

Authors

Sasikala Anbarasan

Janne Janis

Marja Paloheimo

Mikko Laitaoja

Minna Vuolanto

Johanna Karimaki

Pirjo Vainiotalo

Matti Leisola

Ossi Turunen

Affiliations

Soon will be listed here.

Abstract

The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.

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