Water- and Nutrient-dependent Effects of Dietary Restriction on Drosophila Lifespan

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Oct 21

PMID 19841272

Citations 55

Authors

William W Ja

Gil B Carvalho

Brian M Zid

Elizabeth M Mak

Ted Brummel

Seymour Benzer

Affiliations

Soon will be listed here.

Abstract

Dietary restriction (DR) is a widely conserved intervention leading to lifespan extension. Despite considerable effort, the mechanisms underlying DR remain poorly understood. In particular, it remains unclear whether DR prolongs life through conserved mechanisms in different species. Here, we show that, in the most common experimental conditions, lifespan extension by DR is abolished by providing Drosophila with ad libitum water, without altering food intake, indicating that DR, as conventionally studied in flies, is fundamentally different from the phenomenon studied in mammals. We characterize an alternative dietary paradigm that elicits robust lifespan extension irrespective of water availability, and thus likely represents a more relevant model for mammalian DR. Our results support the view that protein:carbohydrate ratio is the main dietary determinant of fly lifespan. These findings have broad implications for the study of lifespan and nutrition.

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References

Longo V, Finch C . Evolutionary medicine: from dwarf model systems to healthy centenarians?. Science. 2003; 299(5611):1342-6. DOI: 10.1126/science.1077991. View

Masoro E . Caloric restriction and aging: controversial issues. J Gerontol A Biol Sci Med Sci. 2006; 61(1):14-9. DOI: 10.1093/gerona/61.1.14. View

Min K, Flatt T, Kulaots I, Tatar M . Counting calories in Drosophila diet restriction. Exp Gerontol. 2006; 42(3):247-51. PMC: 2606145. DOI: 10.1016/j.exger.2006.10.009. View

Guarente L, Kenyon C . Genetic pathways that regulate ageing in model organisms. Nature. 2000; 408(6809):255-62. DOI: 10.1038/35041700. View

Walford R, Mock D, Verdery R, Maccallum T . Calorie restriction in biosphere 2: alterations in physiologic, hematologic, hormonal, and biochemical parameters in humans restricted for a 2-year period. J Gerontol A Biol Sci Med Sci. 2002; 57(6):B211-24. DOI: 10.1093/gerona/57.6.b211. View

Rogina B, Helfand S, Frankel S . Longevity regulation by Drosophila Rpd3 deacetylase and caloric restriction. Science. 2002; 298(5599):1745. DOI: 10.1126/science.1078986. View

Thompson E, Reeder B, Bruce R . Characterization of a method for quantitating food consumption for mutation assays in Drosophila. Environ Mol Mutagen. 1991; 18(1):14-21. DOI: 10.1002/em.2850180104. View

Carey J, Liedo P, Harshman L, Zhang Y, Muller H, Partridge L . Life history response of Mediterranean fruit flies to dietary restriction. Aging Cell. 2003; 1(2):140-8. DOI: 10.1046/j.1474-9728.2002.00019.x. View

Wood J, Rogina B, Lavu S, Howitz K, Helfand S, Tatar M . Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature. 2004; 430(7000):686-9. DOI: 10.1038/nature02789. View

10.

Guarente L, Picard F . Calorie restriction--the SIR2 connection. Cell. 2005; 120(4):473-82. DOI: 10.1016/j.cell.2005.01.029. View

11.

Weindruch R, WALFORD R, Fligiel S, GUTHRIE D . The retardation of aging in mice by dietary restriction: longevity, cancer, immunity and lifetime energy intake. J Nutr. 1986; 116(4):641-54. DOI: 10.1093/jn/116.4.641. View

12.

NELSON J, Gosden R, Felicio L . Effect of dietary restriction on estrous cyclicity and follicular reserves in aging C57BL/6J mice. Biol Reprod. 1985; 32(3):515-22. DOI: 10.1095/biolreprod32.3.515. View

13.

Colman R, Anderson R, Johnson S, Kastman E, Kosmatka K, Beasley T . Caloric restriction delays disease onset and mortality in rhesus monkeys. Science. 2009; 325(5937):201-4. PMC: 2812811. DOI: 10.1126/science.1173635. View

14.

Kapahi P, Zid B, Harper T, Koslover D, Sapin V, Benzer S . Regulation of lifespan in Drosophila by modulation of genes in the TOR signaling pathway. Curr Biol. 2004; 14(10):885-90. PMC: 2754830. DOI: 10.1016/j.cub.2004.03.059. View

15.

Kemnitz J, Weindruch R, Roecker E, Crawford K, Kaufman P, Ershler W . Dietary restriction of adult male rhesus monkeys: design, methodology, and preliminary findings from the first year of study. J Gerontol. 1993; 48(1):B17-26. DOI: 10.1093/geronj/48.1.b17. View

16.

Ja W, Carvalho G, Mak E, de la Rosa N, Fang A, Liong J . Prandiology of Drosophila and the CAFE assay. Proc Natl Acad Sci U S A. 2007; 104(20):8253-6. PMC: 1899109. DOI: 10.1073/pnas.0702726104. View

17.

Mair W, Piper M, Partridge L . Calories do not explain extension of life span by dietary restriction in Drosophila. PLoS Biol. 2005; 3(7):e223. PMC: 1140680. DOI: 10.1371/journal.pbio.0030223. View

18.

Carvalho G, Kapahi P, Anderson D, Benzer S . Allocrine modulation of feeding behavior by the Sex Peptide of Drosophila. Curr Biol. 2006; 16(7):692-6. PMC: 2745344. DOI: 10.1016/j.cub.2006.02.064. View

19.

Clancy D, Gems D, Hafen E, Leevers S, Partridge L . Dietary restriction in long-lived dwarf flies. Science. 2002; 296(5566):319. DOI: 10.1126/science.1069366. View

20.

Carey J, Harshman L, Liedo P, Muller H, Wang J, Zhang Z . Longevity-fertility trade-offs in the tephritid fruit fly, Anastrepha ludens, across dietary-restriction gradients. Aging Cell. 2008; 7(4):470-7. PMC: 2574784. DOI: 10.1111/j.1474-9726.2008.00389.x. View