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Phytochrome-mediated Activation of the Gene for Cytosolic Glutamine-synthetase (GS1) During Imbibition of Photosensitive Lettuce Seeds

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Journal Plant Mol Biol
Date 1990 Aug 1
PMID 1983300
Citations 14
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Abstract

A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glutamine synthetase from other plants. Northern and dot-blot analyses of poly(A)+ RNA extracted from the seeds incubated under various light conditions showed that the activation of the gene for cytosolic glutamine-synthetase during imbibition of lettuce seeds is directly or indirectly regulated by phytochrome.

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