Permethylation and Tandem Mass Spectrometry of Oligosaccharides Having Free Hexosamine: Analysis of the Glycoinositol Phospholipid Anchor Glycan from the Scrapie Prion Protein

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1990 Nov 15

PMID 1981823

Citations 20

Authors

M A Baldwin

N Stahl

L G Reinders

B W Gibson

S B Prusiner

A L Burlingame

Affiliations

Soon will be listed here.

Abstract

Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces specific fragmentation reactions in collision-induced dissociation spectra obtained on a four-sector tandem mass spectrometer, identifying the branching pattern of the PrPSc GPI glycan.

Citing Articles

The dynamics of prion spreading is governed by the interplay between the non-linearities of tissue response and replication kinetics.

Fornara B, Igel A, Beringue V, Martin D, Sibille P, Pujo-Menjouet L iScience. 2024; 27(12):111381.

PMID: 39717079 PMC: 11664133. DOI: 10.1016/j.isci.2024.111381.

GlycoHunter: An Open-Source Software for the Detection and Relative Quantification of INLIGHT-Labeled N-Linked Glycans.

Kalmar J, Garrard K, Muddiman D J Proteome Res. 2021; 20(4):1855-1863.

PMID: 33417767 PMC: 8285078. DOI: 10.1021/acs.jproteome.0c00840.

Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.

Kalmar J, Butler K, Baker E, Muddiman D Anal Bioanal Chem. 2020; 412(27):7569-7579.

PMID: 32844281 PMC: 7541788. DOI: 10.1007/s00216-020-02892-2.

Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.

Callender J, Sevillano A, Soldau K, Kurt T, Schumann T, Pizzo D Neurobiol Dis. 2020; 142:104955.

PMID: 32454127 PMC: 7425694. DOI: 10.1016/j.nbd.2020.104955.

Prion protein glycans reduce intracerebral fibril formation and spongiosis in prion disease.

Sevillano A, Aguilar-Calvo P, Kurt T, Lawrence J, Soldau K, Nam T J Clin Invest. 2020; 130(3):1350-1362.

PMID: 31985492 PMC: 7269597. DOI: 10.1172/JCI131564.