Isolation of High Salinity Stress Tolerant Genes from Pisum Sativum by Random Overexpression in Escherichia Coli and Their Functional Validation

Overview

Journal Plant Signal Behav

Specialty Biology

Date 2009 Oct 10

PMID 19816097

Citations 11

Authors

Amita Joshi

Hung Quang Dang

Neha Vaid

Narendra Tuteja

Affiliations

Soon will be listed here.

Abstract

Salinity stress is one of the major factors which reduce crop plants growth and productivity resulting in significant economic losses worldwide. Therefore, it would be fruitful to isolate and functionally identify new salinity stress-induced genes for understanding the mechanism and developing salinity stress tolerant plants. Based on functional gene screening assay, we have isolated few salinity tolerant genes out of one million Escherichia coli (SOLR) transformants containing pea cDNAs. Sequence analysis of three of these genes revealed homology to Ribosomal-L30E (RPL30E), Chlorophyll-a/b-binding protein (Chla/bBP) and FIDDLEHEAD (FDH). The salinity tolerance of these genes in bacteria was further confirmed by using another strain of E. coli (DH5alpha) transformants. The homology based computational modeling of these proteins suggested the high degree of conservation with the conserved domains of their homologous partners. The reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed that the expression of these cDNAs (except the FDH) was upregulated in pea plants in response to NaCl stress. We observed that there was no significant effect of Li(+) ion on the expression level of these genes, while an increase in response to K(+) ion was observed. Overall, this study provides an evidence for a novel function of these genes in high salinity stress tolerance. The PsFDH showed constitutive expression in planta suggesting that it can be used as constitutively expressed marker gene for salinity stress tolerance in plants. This study brings new direction in identifying novel function of unidentified genes in abiotic stress tolerance without previous knowledge of the genome sequence.

Citing Articles

Cell Mutagenic Autopolyploidy Enhances Salinity Stress Tolerance in Leguminous Crops.

Mangena P Cells. 2023; 12(16).

PMID: 37626892 PMC: 10453822. DOI: 10.3390/cells12162082.

Multi-responses of O-methyltransferase genes to salt stress and fiber development of Gossypium species.

Hafeez A, Ge Q, Zhang Q, Li J, Gong J, Liu R BMC Plant Biol. 2021; 21(1):37.

PMID: 33430775 PMC: 7798291. DOI: 10.1186/s12870-020-02786-6.

De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes.

Das P, Behera B, Chatterjee S, Das B, Mohapatra T PLoS One. 2020; 15(2):e0228199.

PMID: 32040520 PMC: 7010390. DOI: 10.1371/journal.pone.0228199.

Identification of wheat stress-responding genes and TaPR-1-1 function by screening a cDNA yeast library prepared following abiotic stress.

Wang J, Mao X, Wang R, Li A, Zhao G, Zhao J Sci Rep. 2019; 9(1):141.

PMID: 30644420 PMC: 6333785. DOI: 10.1038/s41598-018-37859-y.

Isolation of genes conferring salt tolerance from Piriformospora indica by random overexpression in Escherichia coli.

Gahlot S, Joshi A, Singh P, Tuteja R, Dua M, Jogawat A World J Microbiol Biotechnol. 2015; 31(8):1195-209.

PMID: 25982746 DOI: 10.1007/s11274-015-1867-5.

References

Swire-Clark G, Marcotte Jr W . The wheat LEA protein Em functions as an osmoprotective molecule in Saccharomyces cerevisiae. Plant Mol Biol. 1999; 39(1):117-28. DOI: 10.1023/a:1006106906345. View

Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K . Gene expression profiles during the initial phase of salt stress in rice. Plant Cell. 2001; 13(4):889-905. PMC: 135538. DOI: 10.1105/tpc.13.4.889. View

Sahi C, Agarwal M, Reddy M, Sopory S, Grover A . Isolation and expression analysis of salt stress-associated ESTs from contrasting rice cultivars using a PCR-based subtraction method. Theor Appl Genet. 2003; 106(4):620-8. DOI: 10.1007/s00122-002-1089-8. View

Kreps J, Wu Y, Chang H, Zhu T, Wang X, Harper J . Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol. 2002; 130(4):2129-41. PMC: 166725. DOI: 10.1104/pp.008532. View

Yale J, Bohnert H . Transcript expression in Saccharomyces cerevisiae at high salinity. J Biol Chem. 2001; 276(19):15996-6007. DOI: 10.1074/jbc.M008209200. View

Desper R, Gascuel O . Fast and accurate phylogeny reconstruction algorithms based on the minimum-evolution principle. J Comput Biol. 2002; 9(5):687-705. DOI: 10.1089/106652702761034136. View

Yamada A, Saitoh T, Mimura T, Ozeki Y . Expression of mangrove allene oxide cyclase enhances salt tolerance in Escherichia coli, yeast, and tobacco cells. Plant Cell Physiol. 2002; 43(8):903-10. DOI: 10.1093/pcp/pcf108. View

Kanhonou R, Serrano R, Palau R . A catalytic subunit of the sugar beet protein kinase CK2 is induced by salt stress and increases NaCl tolerance in Saccharomyces cerevisiae. Plant Mol Biol. 2001; 47(5):571-9. DOI: 10.1023/a:1012227913356. View

Rausell A, Kanhonou R, Yenush L, Serrano R, Ros R . The translation initiation factor eIF1A is an important determinant in the tolerance to NaCl stress in yeast and plants. Plant J. 2003; 34(3):257-67. DOI: 10.1046/j.1365-313x.2003.01719.x. View

10.

Pruitt R, Ploense S, Grossniklaus U, Lolle S . FIDDLEHEAD, a gene required to suppress epidermal cell interactions in Arabidopsis, encodes a putative lipid biosynthetic enzyme. Proc Natl Acad Sci U S A. 2000; 97(3):1311-6. PMC: 15605. DOI: 10.1073/pnas.97.3.1311. View

11.

Zhu J . Cell signaling under salt, water and cold stresses. Curr Opin Plant Biol. 2001; 4(5):401-6. DOI: 10.1016/s1369-5266(00)00192-8. View

12.

Xu D, Duan X, Wang B, Hong B, Ho T, Wu R . Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice. Plant Physiol. 1996; 110(1):249-257. PMC: 157716. DOI: 10.1104/pp.110.1.249. View

13.

Gong Q, Li P, Ma S, Rupassara S, Bohnert H . Salinity stress adaptation competence in the extremophile Thellungiella halophila in comparison with its relative Arabidopsis thaliana. Plant J. 2005; 44(5):826-39. DOI: 10.1111/j.1365-313X.2005.02587.x. View

14.

Sung D, Kaplan F, Lee K, Guy C . Acquired tolerance to temperature extremes. Trends Plant Sci. 2003; 8(4):179-87. DOI: 10.1016/S1360-1385(03)00047-5. View

15.

Vanderauwera S, Zimmermann P, Rombauts S, Vandenabeele S, Langebartels C, Gruissem W . Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis reveals a high light-induced transcriptional cluster involved in anthocyanin biosynthesis. Plant Physiol. 2005; 139(2):806-21. PMC: 1255997. DOI: 10.1104/pp.105.065896. View

16.

Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y . Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 2002; 31(3):279-92. DOI: 10.1046/j.1365-313x.2002.01359.x. View

17.

Forment J, Naranjo M, Roldan M, Serrano R, Vicente O . Expression of Arabidopsis SR-like splicing proteins confers salt tolerance to yeast and transgenic plants. Plant J. 2002; 30(5):511-9. DOI: 10.1046/j.1365-313x.2002.01311.x. View

18.

Yamada A, Tsutsumi K, Tanimoto S, Ozeki Y . Plant RelA/SpoT homolog confers salt tolerance in Escherichia coli and Saccharomyces cerevisiae. Plant Cell Physiol. 2003; 44(1):3-9. DOI: 10.1093/pcp/pcg001. View

19.

Mundree S, Whittaker A, Thomson J, Farrant J . An aldose reductase homolog from the resurrection plant Xerophyta viscosa Baker. Planta. 2000; 211(5):693-700. DOI: 10.1007/s004250000331. View

20.

Kultz D . Evolution of the cellular stress proteome: from monophyletic origin to ubiquitous function. J Exp Biol. 2003; 206(Pt 18):3119-24. DOI: 10.1242/jeb.00549. View