The Adhesive Removal Test: a Sensitive Method to Assess Sensorimotor Deficits in Mice

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2009 Oct 3

PMID 19798088

Citations 242

Authors

Valentine Bouet

Michel Boulouard

Jerome Toutain

Didier Divoux

Myriam Bernaudin

Pascale Schumann-Bard

Thomas Freret

Affiliations

Soon will be listed here.

Abstract

Long-term functional deficits after a brain injury are difficult to assess in the mouse. If no deficit is observed, researchers could conclude either that the animal has fully recovered or that the tests they used were not appropriate or sensitive enough to the modality of the deficits. We present here a detailed protocol describing how to conduct an adhesive removal test for this species. It consists of applying adhesive tape on each forepaw of the animal and measuring the time-to-contact and the time-to-remove them. This behavior implies correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove). To decrease interindividual differences, we recommend a training session (1 week, 1 trial per day) before surgical procedures so that mice to reach optimal performances.

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