Targeted Transduction Via CD4 by a Lentiviral Vector Uses a Clathrin-mediated Entry Pathway

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2009 Oct 2

PMID 19793825

Citations 12

Authors

Min Liang

Kouki Morizono

Nonia Pariente

Masakazu Kamata

Benhur Lee

Irvin S Y Chen

Affiliations

Soon will be listed here.

Abstract

We recently developed a novel targeting Sindbis virus envelope pseudotyped lentiviral vector, 2.2ZZ, which acquires specific transduction capacity by antibody conjugation and binding with specific antigens on the surface of targeted cells. Here we characterize the virological properties of this vector by examining its targeting to CD4 antigen. Our results show that entry is dependent on CD4 cell surface density and occurs via the clathrin-mediated endocytic pathway. These findings provide insight into the mechanism of infection by a new viral vector with combined properties of Sindbis virus and lentiviruses and infectivity conferred by monoclonal antibody-ligand interactions.

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