Identification of the Transforming Activity of Indian Hedgehog by Retroviral Expression Screening

Overview

Journal Cancer Sci

Specialty Oncology

Date 2009 Oct 2

PMID 19793350

Citations 1

Authors

Hisashi Hatanaka

Shuji Takada

Mamiko Tsukui

Young Lim Choi

Kentaro Kurashina

Manabu Soda

Yoshihiro Yamashita

Hidenori Haruta

Toru Hamada

Kiichi Tamada

Yoshinori Hosoya

Naohiro Sata

Hideo Nagai

Yoshikazu Yasuda

Kentaro Sugano

Hiroyuki Mano

Affiliations

Soon will be listed here.

Abstract

To identify novel cancer-promoting genes in biliary tract cancer (BTC), we constructed a retroviral cDNA expression library from a clinical specimen of BTC with anomalous pancreaticobiliary duct junction (APBDJ), and used the library for a focus formation assay with 3T3 fibroblasts. One of the cDNAs rescued from transformed foci was found to encode Indian hedgehog homolog (IHH). The oncogenic potential of IHH was confirmed both in vitro with the focus formation assay and in vivo with a tumorigenicity assay in nude mice. The isolated IHH cDNA had no sequence alterations, suggesting that upregulation of IHH expression may contribute to malignant transformation. Quantitation of IHH mRNA among clinical specimens has revealed that the expression level of IHH in BTC with APBDJ is higher than that in BTC without APBDJ and than in non-cancerous biliary tissues. Our data thus implicate a direct role of IHH in the carcinogenesis of BTC with APBDJ.

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