A Large Insert Thellungiella Halophila BIBAC Library for Genomics and Identification of Stress Tolerance Genes

Overview

Journal Plant Mol Biol

Date 2009 Sep 30

PMID 19787433

Citations 7

Authors

Weiquan Wang

Yaorong Wu

Yin Li

Jiaying Xie

Zhonghui Zhang

Zhiyong Deng

Yiyue Zhang

Cuiping Yang

Jianbin Lai

Huawei Zhang

Hongyan Bao

Sanyuan Tang

Chengwei Yang

Peng Gao

Guixian Xia

Huishan Guo

Qi Xie

Affiliations

Soon will be listed here.

Abstract

Salt cress (Thellungiella halophila), a salt-tolerant relative of Arabidopsis, has turned to be an important model plant for studying abiotic stress tolerance. One binary bacterial artificial chromosome (BIBAC) library was constructed which represents the first plant-transformation-competent large-insert DNA library generated for Thellungiella halophila. The BIBAC library was constructed in BamHI site of binary vector pBIBAC2 by ligation of partial digested nuclear DNA of Thellungiella halophila. This library consists of 23,040 clones with an average insert size of 75 kb, and covers 4x Thellungiella halophila haploid genomes. BIBAC clones which contain inserts over 50 kb were selected and transformed into Arabidopsis for salt tolerant plant screening. One transgenic line was found to be more salt tolerant than wild type plants from the screen of 200 lines. It was demonstrated that the library contains candidates of stress tolerance genes and the approach is suitable for the transformation of stress susceptible plants for genetic improvement.

Citing Articles

Halophytism: What Have We Learnt From Relative Model Systems?.

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An assessment of transgenomics as a tool for gene discovery in Populus euphratica Oliv.

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector.

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Salt Induces Features of a Dormancy-Like State in Seeds of Eutrema (Thellungiella) salsugineum, a Halophytic Relative of Arabidopsis.

Kazachkova Y, Khan A, Acuna T, Lopez-Diaz I, Carrera E, Khozin-Goldberg I Front Plant Sci. 2016; 7:1071.

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