The Influence of Artificial Colonization with E. Coli Strain O83 on the Intestinal Flora in Infants

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 1990 Jan 1

PMID 1976579

Authors

M Slavikova

R Lodinova-Zadnikova

L A Hanson

I Adlerberth

B Carlsson

A E Wold

C Scanborg Eden

Affiliations

Soon will be listed here.

Abstract

Dominant bacterial strains present in stool (with particular emphasis on E. coli strains) were examined in 4 groups of healthy infants: breast-fed and bottle-fed, colonized with E. coli O83, and control (non-colonized) breast-fed and bottle-fed newborns. The presence of fimbriae was examined by hemagglutination, the P-fimbriae-bearing strains were tested by the PPA latex test. In addition, adherence to cell line HT-29 and serotyping was performed in selected strains. The E. coli strain O83 was found to possess type 1 fimbriae. Fewer bacterial strains possessing type 1 fimbriae were found in E. coli O83-colonized infants (except the O83 serotype) than in control infants. The E. coli O83 strain colonized significantly better the breast-fed than the bottle-fed infants; its higher adherence activity was demonstrated even in cell line HT-29. Finally, colonization with E. coli O83 influenced the character of microbial intestinal flora: the frequency of positive E. coli isolates was significantly higher in colonized (both breast- and bottle-fed) than noncolonized infants.

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