A Switchable Light-input, Light-output System Modelled and Constructed in Yeast

Overview

Journal J Biol Eng

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2009 Sep 19

PMID 19761615

Citations 13

Authors

Oxana Sorokina

Anita Kapus

Kata Terecskei

Laura E Dixon

Laszlo Kozma-Bognar

Ferenc Nagy

Andrew J Millar

Affiliations

Soon will be listed here.

Abstract

Background: Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.

Results: The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.

Conclusion: Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.

Citing Articles

Optogenetic control of gene expression in the cyanobacterium sp. PCC 7002.

Forbes L, Papanatsiou M, Palombo A, Christie J, Amtmann A Front Bioeng Biotechnol. 2025; 12:1529022.

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PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in .

Machens F, Ran G, Ruehmkorff C, Meyer Auf der Heyde J, Mueller-Roeber B, Hochrein L ACS Synth Biol. 2023; 12(4):1046-1057.

PMID: 37014634 PMC: 10127447. DOI: 10.1021/acssynbio.2c00517.

Expanding the molecular versatility of an optogenetic switch in yeast.

Figueroa D, Baeza C, Ruiz D, Inzunza C, Romero A, Toro R Front Bioeng Biotechnol. 2022; 10:1029217.

PMID: 36457859 PMC: 9705753. DOI: 10.3389/fbioe.2022.1029217.

A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners.

Li H, Qin X, Song P, Han R, Li J aBIOTECH. 2022; 2(2):105-116.

PMID: 36304755 PMC: 9590525. DOI: 10.1007/s42994-021-00034-5.

Optogenetics for transcriptional programming and genetic engineering.

Lan T, He L, Huang Y, Zhou Y Trends Genet. 2022; 38(12):1253-1270.

PMID: 35738948 PMC: 10484296. DOI: 10.1016/j.tig.2022.05.014.

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