Role of SIP30 in the Development and Maintenance of Peripheral Nerve Injury-induced Neuropathic Pain

Overview

Journal Pain

Specialties Neurology
Psychiatry

Date 2009 Sep 15

PMID 19748740

Citations 16

Authors

Yu-Qiu Zhang

Ning Guo

Guangdun Peng

Xidao Wang

Mei Han

Jeremy Raincrow

Chi-Hua Chiu

Lique M Coolen

Robert J Wenthold

Zhi-Qi Zhao

Naihe Jing

Lei Yu

Affiliations

Soon will be listed here.

Abstract

Using the chronic constriction injury (CCI) model of neuropathic pain, we profiled gene expression in the rat spinal cord, and identified SIP30 as a gene whose expression was elevated after CCI. SIP30 was previously shown to interact with SNAP25, but whose function was otherwise unknown. We now show that in the spinal cord, SIP30 was present in the dorsal horn laminae where the peripheral nociceptive inputs first synapse, co-localizing with nociception-related neuropeptides CGRP and substance P. With the onset of neuropathic pain after CCI surgery, SIP30 mRNA and protein levels increased in the ipsilateral side of the spinal cord, suggesting a potential association between SIP30 and neuropathic pain. When CCI-upregulated SIP30 was inhibited by intrathecal antisense oligonucleotide administration, neuropathic pain was attenuated. This neuropathic pain-reducing effect was observed both during neuropathic pain onset following CCI, and after neuropathic pain was fully established, implicating SIP30 involvement in the development and maintenance phases of neuropathic pain. Using a secretion assay in PC12 cells, anti-SIP30 siRNA decreased the total pool of synaptic vesicles available for exocytosis, pointing to a potential function for SIP30. These results suggest a role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain.

Citing Articles

Phylogenetic analysis of mammalian SIP30 sequences indicating accelerated adaptation of functional domain in primates.

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SIP30 involvement in vesicle exocytosis from PC12 cells.

Guo N, Yu L Biochem Biophys Rep. 2024; 37:101614.

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