Mitochondrial Respiration in Myocardial Biopsy Samples As a Criterion of Postischemic Recovery of the Cardiac Contractility

Overview

Journal Basic Res Cardiol

Specialty Cardiology & Vascular Diseases

Date 1990 May 1

PMID 1974419

Citations 2

Authors

V I VEKSLER

A N Khatkevich

G V Elizarova

V I Kapelko

Affiliations

Soon will be listed here.

Abstract

Isolated perfused guinea pig hearts were arrested by a high K+ cardioplegic solution containing (PG group) or lacking (control group) 10 mM phosphocreatine + 15 mM glutamate. Total normothermic ischemia lasted 45 min followed by 30 min reperfusion. Mitochondrial respiration in the absence and presence of different concentrations of ADP and creatine was studied in biopsy samples (6-8 mg) after saponin treatment. The samples were taken before and after ischemia, as well as after the reperfusion period. A slightly better relative recovery of developed pressure (RRDP) in PG group was associated with higher mitochondrial acceptor control ratio after reperfusion (5.74 +/- 0.32 vs. 4.54 +/- 0.21 in PG and control groups, resp., p less than 0.01). When the results obtained in both groups were treated together, tight correlations between the pre- or postischemic mitochondrial state and RRDP were revealed. Higher values of RRDP were found for the hearts with lower preischemic values of (low ADP + creatine)-stimulation of mitochondrial respiration (r = -0.57, p less than 0.01). Relative changes in this mitochondrial parameter during ischemic period were in a good correlation with the RRDP (r = 0.82, p less than 0.001). The data suggest that the study of the mitochondrial function in myocardial biopsy samples before ischemia and reperfusion could provide a useful information for the prognosis of cardiac function recovery.

Citing Articles

Early ischemia-induced alterations of the outer mitochondrial membrane and the intermembrane space: a potential cause for altered energy transfer in cardiac muscle?.

Rossi A, Kay L, Saks V Mol Cell Biochem. 1998; 184(1-2):401-8.

PMID: 9746334

The effects of ischemia and experimental conditions on the respiration rate of cardiac fibers.

Toleikis A, Majiene D, Trumbeckaite S, Dagys A Mol Cell Biochem. 1997; 174(1-2):87-90.

PMID: 9309670

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