Stretched Extracellular Matrix Proteins Turn Fouling and Are Functionally Rescued by the Chaperones Albumin and Casein

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2009 Sep 12

PMID 19743815

Citations 19

Authors

William C Little

Ruth Schwartlander

Michael L Smith

Delphine Gourdon

Viola Vogel

Affiliations

Soon will be listed here.

Abstract

While evidence is mounting that cells exploit protein unfolding for mechanochemical signal conversion (mechanotransduction), what mechanisms are in place to deal with the unwanted consequences of exposing hydrophobic residues upon force-induced protein unfolding? Here, we show that mechanical chaperones exist that can transiently bind to hydrophobic residues that are freshly exposed by mechanical force. The stretch-upregulated binding of albumin or casein to fibronectin fibers is reversible and does not inhibit fiber contraction once the tension is released.

Citing Articles

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Pankova D, Jiang Y, Chatzifrangkeskou M, Vendrell I, Buzzelli J, Ryan A EMBO J. 2019; 38(13):e100532.

PMID: 31268606 PMC: 6600643. DOI: 10.15252/embj.2018100532.

Fibronectin fiber creep under constant force loading.

Bradshaw M, Hoffmann G, Wong J, Smith M Acta Biomater. 2019; 88:78-85.

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Novel peptide probes to assess the tensional state of fibronectin fibers in cancer.

Arnoldini S, Moscaroli A, Chabria M, Hilbert M, Hertig S, Schibli R Nat Commun. 2017; 8(1):1793.

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Walking the Line: A Fibronectin Fiber-Guided Assay to Probe Early Steps of (Lymph)angiogenesis.

Mitsi M, Schulz M, Gousopoulos E, Ochsenbein A, Detmar M, Vogel V PLoS One. 2015; 10(12):e0145210.

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