Endonuclease for Apurinic Sites in Yeast. Comparison of the Enzyme Activity in the Wild Type and in Rad Mutants of Saccharomyces Cerevisiae to MNS

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Jul 20

PMID 197392

Citations 6

Authors

E Chlebowicz

W J Jachymczyk

Affiliations

Soon will be listed here.

Abstract

It was found that yeast cells contain an endonuclease specific for apurinic sites in DNA which has no effect on DNA with normal strands or on strands with alkylated sites. The enzyme activity was studied in the RAD strain and in rad 6, rad 18-2 and rad 21 mutants, all very sensitive to MMS, as compared to the wild type. The level of endonuclease activity does not differ much between the tested strains, regardless of their differences in susceptibility to MMS. The enzyme activity is not induced by pretreatment of the cells with this mutagen.

Citing Articles

DNA repair in Saccharomyces cerevisiae: purification and characterization of apurinic endonucleases.

Armel P, Wallace S J Bacteriol. 1984; 160(3):895-902.

PMID: 6389517 PMC: 215794. DOI: 10.1128/jb.160.3.895-902.1984.

Repair of gamma-ray induced DNA strand breaks in the radiation-sensitive mutant rad18-2 of Saccharomyces cerevisiae.

Mowat M, Jachymczyk W, Hastings P, VON Borstel R Mol Gen Genet. 1983; 189(2):256-62.

PMID: 6343790 DOI: 10.1007/BF00337814.

Apurinic endonuclease from Saccharomyces cerevisiae.

Thielmann H, Hess U Biochem J. 1981; 195(2):407-17.

PMID: 6274306 PMC: 1162904. DOI: 10.1042/bj1950407.

The yeast rad18 mutator specifically increases G.C----T.A transversions without reducing correction of G-A or C-T mismatches to G.C pairs.

Kunz B, Kang X, Kohalmi L Mol Cell Biol. 1991; 11(1):218-25.

PMID: 1986222 PMC: 359612. DOI: 10.1128/mcb.11.1.218-225.1991.

Elimination of the yeast RAD6 ubiquitin conjugase enhances base-pair transitions and G.C----T.A transversions as well as transposition of the Ty element: implications for the control of spontaneous mutation.

Kang X, Yadao F, Gietz R, Kunz B Genetics. 1992; 130(2):285-94.

PMID: 1311695 PMC: 1204849. DOI: 10.1093/genetics/130.2.285.

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