Phenylalanine As Substrate for Tyrosine Hydroxylase in Bovine Adrenal Chromaffin Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1990 Jun 1

PMID 1973034

Citations 6

Authors

M H Fukami

J Haavik

T Flatmark

Affiliations

Soon will be listed here.

Abstract

Incubation of bovine chromaffin cells with L-[14C]phenylalanine resulted in label accumulation in catecholamines at about 30% of the rate seen with L-tyrosine as precursor. Studies with purified tyrosine hydroxylase (EC 1.14.16.2) showed that the enzyme catalysed the hydroxylation of L-phenylalanine first to L-p-tyrosine and then to 3,4-dihydroxyphenylalanine (DOPA). No evidence for a significant involvement of an L-m-tyrosine intermediate in DOPA formation was found.

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