The GTPase Rab3b/3c-positive Recycling Vesicles Are Involved in Cross-presentation in Dendritic Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Sep 1

PMID 19717423

Citations 33

Authors

Liyun Zou

Jingran Zhou

Jinyu Zhang

Jingyi Li

Na Liu

Linlin Chai

Na Li

Ting Liu

Liqi Li

Zhunyi Xie

Hongli Liu

Ying Wan

Yuzhang Wu

Affiliations

Soon will be listed here.

Abstract

Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process, but the underlying molecular mechanisms remain to be thoroughly investigated. In this study, we examined the effect of siRNA-mediated knockdown of 57 Rab GTPases, the key regulators of membrane trafficking, on antigen cross-presentation. Twelve Rab GTPases were identified to be associated with antigen cross-presentation, and Rab3b/3c was indicated to be colocalized with MHC class I molecules at perinuclear tubular structure. Tracing with fluorescence protein-tagged beta(2)-microglobulin demonstrated that the MHC class I molecules were internalized from the plasma membrane to Rab3b/3c-positive compartments, which were also colocalized with the internalized transferrin. Moreover, depletion of Rab3b/3c strongly reduced the fast phase recycling rate of transferrin receptors. Furthermore, the Rab3b/3c-positive compartments were colocalized with a fraction of Rab27a at a juxtaposition of phagosomes. Together, these data demonstrate that Rab3b/3c-positive recycling vesicles are involved in and may constitute one of the recycling compartments in exogenous antigen cross-presentation.

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