Downstream Targets of WRKY33

Overview

Journal Plant Signal Behav

Specialty Biology

Date 2009 Aug 26

PMID 19704444

Citations 12

Authors

Klaus Petersen

Berthe Katrine Fiil

John Mundy

Morten Petersen

Affiliations

Soon will be listed here.

Abstract

Innate immunity signaling pathways in both animals and plants are regulated by mitogen-activated protein kinase (MAPK) cascades. In a recent publication we show that MPK4 and its substrate MKS1 interact with WRKY33 in vivo, and that WRKY33 is released from complexes with MPK4 upon infection. Transcriptome analysis of a wrky33 loss-of-function mutant identified a subset of defense-related genes as putative targets of WRKY33. These genes include PAD3 and CYP71A13, which encode cytochrome P450 monoxygenases required for synthesis of the antimicrobial phytoalexin camalexin. Chromatin immunoprecipitation confirmed that WRKY33 bound the promoter of PAD3 when plants were inoculated with pathogens. Here we further discuss the involvement of two other targets of WRKY33, NUDT6 and ROF2 in defense responses against invading pathogens.

Citing Articles

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Ambivalent response in pathogen defense: A double-edged sword?.

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Inactivation of UDP-Glucose Sterol Glucosyltransferases Enhances Resistance to .

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References

Hubert D, Tornero P, Belkhadir Y, Krishna P, Takahashi A, Shirasu K . Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein. EMBO J. 2003; 22(21):5679-89. PMC: 275404. DOI: 10.1093/emboj/cdg547. View

Ogawa T, Ueda Y, Yoshimura K, Shigeoka S . Comprehensive analysis of cytosolic Nudix hydrolases in Arabidopsis thaliana. J Biol Chem. 2005; 280(26):25277-83. DOI: 10.1074/jbc.M503536200. View

Qiu J, Fiil B, Petersen K, Nielsen H, Botanga C, Thorgrimsen S . Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus. EMBO J. 2008; 27(16):2214-21. PMC: 2519101. DOI: 10.1038/emboj.2008.147. View

Suarez-Rodriguez M, Adams-Phillips L, Liu Y, Wang H, Su S, Jester P . MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants. Plant Physiol. 2006; 143(2):661-9. PMC: 1803745. DOI: 10.1104/pp.106.091389. View

Zheng Z, Qamar S, Chen Z, Mengiste T . Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant J. 2006; 48(4):592-605. DOI: 10.1111/j.1365-313X.2006.02901.x. View

Petersen M, Brodersen P, Naested H, Andreasson E, Lindhart U, Johansen B . Arabidopsis map kinase 4 negatively regulates systemic acquired resistance. Cell. 2001; 103(7):1111-20. DOI: 10.1016/s0092-8674(00)00213-0. View

Ge X, Li G, Wang S, Zhu H, Zhu T, Wang X . AtNUDT7, a negative regulator of basal immunity in Arabidopsis, modulates two distinct defense response pathways and is involved in maintaining redox homeostasis. Plant Physiol. 2007; 145(1):204-15. PMC: 1976571. DOI: 10.1104/pp.107.103374. View

Nafisi M, Goregaoker S, Botanga C, Glawischnig E, Olsen C, Halkier B . Arabidopsis cytochrome P450 monooxygenase 71A13 catalyzes the conversion of indole-3-acetaldoxime in camalexin synthesis. Plant Cell. 2007; 19(6):2039-52. PMC: 1955726. DOI: 10.1105/tpc.107.051383. View

Aviezer-Hagai K, Skovorodnikova J, Galigniana M, Farchi-Pisanty O, Maayan E, Bocovza S . Arabidopsis immunophilins ROF1 (AtFKBP62) and ROF2 (AtFKBP65) exhibit tissue specificity, are heat-stress induced, and bind HSP90. Plant Mol Biol. 2006; 63(2):237-55. DOI: 10.1007/s11103-006-9085-z. View

10.

Qiu J, Zhou L, Yun B, Nielsen H, Fiil B, Petersen K . Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1. Plant Physiol. 2008; 148(1):212-22. PMC: 2528087. DOI: 10.1104/pp.108.120006. View

11.

Nakagami H, Pitzschke A, Hirt H . Emerging MAP kinase pathways in plant stress signalling. Trends Plant Sci. 2005; 10(7):339-46. DOI: 10.1016/j.tplants.2005.05.009. View

12.

Jonak C, Okresz L, Bogre L, Hirt H . Complexity, cross talk and integration of plant MAP kinase signalling. Curr Opin Plant Biol. 2002; 5(5):415-24. DOI: 10.1016/s1369-5266(02)00285-6. View

13.

Jones J, Dangl J . The plant immune system. Nature. 2006; 444(7117):323-9. DOI: 10.1038/nature05286. View

14.

Brodersen P, Petersen M, Nielsen H, Zhu S, Newman M, Shokat K . Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4. Plant J. 2006; 47(4):532-46. DOI: 10.1111/j.1365-313X.2006.02806.x. View

15.

Harrar Y, Bellini C, Faure J . FKBPs: at the crossroads of folding and transduction. Trends Plant Sci. 2001; 6(9):426-31. DOI: 10.1016/s1360-1385(01)02044-1. View

16.

Andreasson E, Jenkins T, Brodersen P, Thorgrimsen S, Petersen N, Zhu S . The MAP kinase substrate MKS1 is a regulator of plant defense responses. EMBO J. 2005; 24(14):2579-89. PMC: 1176463. DOI: 10.1038/sj.emboj.7600737. View

17.

Chisholm S, Coaker G, Day B, Staskawicz B . Host-microbe interactions: shaping the evolution of the plant immune response. Cell. 2006; 124(4):803-14. DOI: 10.1016/j.cell.2006.02.008. View

18.

Caspersen M, Qiu J, Zhang X, Andreasson E, Naested H, Mundy J . Phosphorylation sites of Arabidopsis MAP kinase substrate 1 (MKS1). Biochim Biophys Acta. 2007; 1774(9):1156-63. DOI: 10.1016/j.bbapap.2007.07.002. View

19.

Bartsch M, Gobbato E, Bednarek P, Debey S, Schultze J, Bautor J . Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7. Plant Cell. 2006; 18(4):1038-51. PMC: 1425861. DOI: 10.1105/tpc.105.039982. View