Different Patterns of Intestinal Response to Injury After Arterial, Venous or Arteriovenous Occlusion in Rats

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2009 Aug 25

PMID 19701970

Citations 11

Authors

Francisco Javier Guzman-de la Garza

Carlos Rodrigo Camara-Lemarroy

Gabriela Alarcon-Galvan

Paula Cordero-Perez

Linda Elsa Munoz-Espinosa

Nancy Esthela Fernandez-Garza

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the differences in injury patterns caused by arterial, venous or arteriovenous mesenteric occlusion.

Methods: Male Wistar rats were separated equally into four groups. Occlusion was performed by clamping the superior mesenteric artery (A), the mesenteric vein (V) or both (AV) for 30 min, followed by 60 min of reperfusion. A control group received sham surgery only. Intestinal sections were examined for histological damage and serum tumor necrosis factor-alpha (TNF-alpha), endothelin-1 (ET-1), P-selectin, antithrombin III (ATIII) and soluble intracellular adhesion molecule-1 (ICAM-1) concentrations were measured.

Results: All groups showed significant mucosal injury compared to controls. Furthermore, mucosal injury was significantly more severe in the V and AV groups compared to the A group (3.6 +/- 0.55, 3.4 +/- 0.55 and 2 +/- 0.71, respectively P = 0.01). ICAM-1 was similarly elevated in all groups, with no significant differences between the groups. P-selectin levels were significantly elevated in the V and AV groups but not the A group (1.4 +/- 0.5 ng/mL, 2.52 +/- 0.9 ng/mL and 0.02 +/- 0.01 ng/mL, respectively, P = 0.01) and ET-1 was significantly elevated in the A and V groups but not the AV group (0.32 +/- 0.04 pg/mL, 0.36 +/- 0.05 pg/mL and 0.29 +/- 0.03 pg/mL, respectively, P = 0.01) compared to sham controls. ATIII levels were markedly depleted in the V and AV groups, but not in the A group (29.1 +/- 5.2 pg/mL, 31.4 +/- 21.8 pg/mL and 55.8 +/- 35.6 pg/mL, respectively, P = 0.01), compared to controls. Serum TNF-alpha was significantly increased in all groups compared to sham controls (1.32 +/- 0.87 ng/mL, 1.79 +/- 0.20 ng/mL and 4.4 +/- 0.69 ng/mL, for groups A, V and AV, respectively, P = 0.01), with higher values in the AV group.

Conclusion: Different patterns of response to ischemia/reperfusion are associated with venous, arterial or arteriovenous occlusion. Venous and arteriovenous occlusion was associated with the most severe alterations.

Citing Articles

Real-time quantification of intestinal perfusion and arterial versus venous occlusion using laser speckle contrast imaging in porcine model.

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Prediction factors for ischemia of closed-loop small intestinal obstruction.

Pavlidis E, Pavlidis T World J Gastrointest Surg. 2022; 14(9):1086-1088.

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Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine.

Amic F, Drmic D, Bilic Z, Krezic I, Zizek H, Peklic M World J Gastroenterol. 2019; 24(47):5366-5378.

PMID: 30598581 PMC: 6305534. DOI: 10.3748/wjg.v24.i47.5366.

Discrimination between arterial and venous bowel ischemia by computer-assisted analysis of the fluorescent signal.

Quero G, Lapergola A, Barberio M, Seeliger B, Saccomandi P, Guerriero L Surg Endosc. 2018; 33(6):1988-1997.

PMID: 30327913 DOI: 10.1007/s00464-018-6512-6.

Bacterial translocation and endotoxemia after pringle maneuver in cirrhotic rats.

Su Y, Pan H, Guo Z, Zhou W, Zhang B Dig Dis Sci. 2014; 60(2):414-9.

PMID: 25287003 DOI: 10.1007/s10620-014-3381-2.

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