Antioxidant Activity of Some Plant Extracts Towards Xanthine Oxidase, Lipoxygenase and Tyrosinase

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2009 Aug 25

PMID 19701137

Citations 23

Authors

Chin-Hui Chen

Hsiu-Chen Chan

Yi-Tsu Chu

Hsin-Yi Ho

Pi-Yu Chen

Tzong-Huei Lee

Ching-Kuo Lee

Affiliations

Soon will be listed here.

Abstract

Natural products have the potential to be developed into new drugs for the treatment of various diseases. The aim of the present study was to screen the antioxidant activities of some common edible fruits, garden plants and medicinal plants indigenous to Taiwan. This was performed by assessing the activities of lipoxygenase, xanthine oxidase and tyrosinase following incubation with extracts from these plants. A further aim was to use HPLC-DAD and tyrosinase to chromatographically identify the antioxidative constituents obtained from an extract exhibiting strong antioxidative properties. The acetone extracts of 27 cultivated plant species from Taiwan were tested for antioxidant activities towards xanthine oxidase, tyrosinase and lipoxygenase using spectrophotometric assays. Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea showed the highest xanthine oxidase inhibitory activities. Camellia sinensis, Rhodiola rosea, and Koelreuteria henryi exhibited good tyrosinase inhibitory activities and potent anti-lipoxygenase activities. As Koelreuteria henryi had notable significant inhibitory activities towards xanthine oxidase, tyrosinase, and lipoxygenase, it was further tested with tyrosinase and HPLC-DAD. The results from this part of the study revealed that the more powerful the antioxidant capability of the extracted component, the greater the decrease in peak height obtained after reacting with tyrosinase. Additional studies are warranted to further characterize the compounds responsible for the antioxidant properties of the examined extracts.

Citing Articles

Antioxidant, Enzyme Inhibitory, and Protective Effect of Extract.

Daescu A, Nistor M, Nicolescu A, Pop R, Bunea A, Rugina D Plants (Basel). 2024; 13(10).

PMID: 38794418 PMC: 11125170. DOI: 10.3390/plants13101347.

Potential Effects of Garlic ( L.) on the Performance, Immunity, Gut Health, Anti-Oxidant Status, Blood Parameters, and Intestinal Microbiota of Poultry: An Updated Comprehensive Review.

Abd El-Ghany W Animals (Basel). 2024; 14(3).

PMID: 38338142 PMC: 10854602. DOI: 10.3390/ani14030498.

Phytochemical analysis, GC-MS profile and determination of antibacterial, antifungal, anti-inflammatory, antioxidant activities of peel and seeds extracts (chloroform and ethyl acetate) of L.

Tavanappanavar A, Mulla S, Seth C, Bagewadi Z, Rahamathulla M, Ahmed M Saudi J Biol Sci. 2023; 31(1):103878.

PMID: 38125735 PMC: 10730893. DOI: 10.1016/j.sjbs.2023.103878.

Eucalyptus torquata L. flowers: a comprehensive study reporting their metabolites profiling and anti-gouty arthritis potential.

Ashour R, El-Shiekh R, Sobeh M, Abdelfattah M, Abdel-Aziz M, Okba M Sci Rep. 2023; 13(1):18682.

PMID: 37907626 PMC: 10618445. DOI: 10.1038/s41598-023-45499-0.

Effects of garlic and lemon essential oils on performance, digestibility, plasma metabolite, and intestinal health in broilers under environmental heat stress.

Elbaz A, Ashmawy E, Salama A, Abdel-Moneim A, Badri F, Thabet H BMC Vet Res. 2022; 18(1):430.

PMID: 36503512 PMC: 9743731. DOI: 10.1186/s12917-022-03530-y.

References

Mates J, Perez-Gomez C, Nunez de Castro I . Antioxidant enzymes and human diseases. Clin Biochem. 2000; 32(8):595-603. DOI: 10.1016/s0009-9120(99)00075-2. View

Pinnell S . Cutaneous photodamage, oxidative stress, and topical antioxidant protection. J Am Acad Dermatol. 2003; 48(1):1-19; quiz 20-2. DOI: 10.1067/mjd.2003.16. View

Rackova L, Oblozinsky M, Kostalova D, Kettmann V, Bezakova L . Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. J Inflamm (Lond). 2007; 4:15. PMC: 1994948. DOI: 10.1186/1476-9255-4-15. View

No J, Soung D, Kim Y, Shim K, Jun Y, Rhee S . Inhibition of tyrosinase by green tea components. Life Sci. 1999; 65(21):PL241-6. DOI: 10.1016/s0024-3205(99)00492-0. View

Mayne S . Antioxidant nutrients and chronic disease: use of biomarkers of exposure and oxidative stress status in epidemiologic research. J Nutr. 2003; 133 Suppl 3:933S-940S. DOI: 10.1093/jn/133.3.933S. View

Nijveldt R, van Nood E, van Hoorn D, Boelens P, van Norren K, Van Leeuwen P . Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74(4):418-25. DOI: 10.1093/ajcn/74.4.418. View

Cui T, Nakamura K, Tian S, Kayahara H, Tian Y . Polyphenolic content and physiological activities of Chinese hawthorn extracts. Biosci Biotechnol Biochem. 2006; 70(12):2948-56. DOI: 10.1271/bbb.60361. View

Halliwell B . Antioxidants in human health and disease. Annu Rev Nutr. 1996; 16:33-50. DOI: 10.1146/annurev.nu.16.070196.000341. View

Lee M, Jiang C, Juan S, Lin R, Hou W . Antioxidant and heme oxygenase-1 (HO-1)-induced effects of selected Taiwanese plants. Fitoterapia. 2006; 77(2):109-15. DOI: 10.1016/j.fitote.2005.11.012. View

10.

Zheng W, Wang S . Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001; 49(11):5165-70. DOI: 10.1021/jf010697n. View

11.

Sturm R, Teasdale R, Box N . Human pigmentation genes: identification, structure and consequences of polymorphic variation. Gene. 2001; 277(1-2):49-62. DOI: 10.1016/s0378-1119(01)00694-1. View

12.

Gerasimidis K, Fillou D, Babatzimcpoulou M, Tassou K, Katsikas H . Preparation of an edible cottonseed protein concentrate and evaluation of its functional properties. Int J Food Sci Nutr. 2007; 58(6):486-90. DOI: 10.1080/09637480701288488. View

13.

Noguchi N, Niki E . Phenolic antioxidants: a rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radic Biol Med. 2000; 28(10):1538-46. DOI: 10.1016/s0891-5849(00)00256-2. View

14.

Pieroni A, Janiak V, Durr C, Ludeke S, Trachsel E, Heinrich M . In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in southern Italy. Phytother Res. 2002; 16(5):467-73. DOI: 10.1002/ptr.1243. View

15.

Masuda T, Inaba Y, Maekawa T, Takeda Y, Yamaguchi H, Nakamoto K . Simple detection method of powerful antiradical compounds in the raw extract of plants and its application for the identification of antiradical plant constituents. J Agric Food Chem. 2003; 51(7):1831-8. DOI: 10.1021/jf026112m. View

16.

Lyckander I, Malterud K . Lipophilic flavonoids from Orthosiphon spicatus prevent oxidative inactivation of 15-lipoxygenase. Prostaglandins Leukot Essent Fatty Acids. 1996; 54(4):239-46. DOI: 10.1016/s0952-3278(96)90054-x. View

17.

Hout S, Chea A, Bun S, Elias R, Gasquet M, Timon-David P . Screening of selected indigenous plants of Cambodia for antiplasmodial activity. J Ethnopharmacol. 2006; 107(1):12-8. DOI: 10.1016/j.jep.2006.01.028. View

18.

Eloff J . Which extractant should be used for the screening and isolation of antimicrobial components from plants?. J Ethnopharmacol. 1998; 60(1):1-8. DOI: 10.1016/s0378-8741(97)00123-2. View

19.

Bandoniene D, Murkovic M . On-line HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.). J Agric Food Chem. 2002; 50(9):2482-7. DOI: 10.1021/jf011475s. View