Nucleocytoplasmic Transport of Alp7/TACC Organizes Spatiotemporal Microtubule Formation in Fission Yeast

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2009 Aug 22

PMID 19696784

Citations 19

Authors

Masamitsu Sato

Naoyuki Okada

Yasutaka Kakui

Masayuki Yamamoto

Minoru Yoshida

Takashi Toda

Affiliations

Soon will be listed here.

Abstract

Ran GTPase activates several target molecules to induce microtubule formation around the chromosomes and centrosomes. In fission yeast, in which the nuclear envelope does not break down during mitosis, Ran targets the centrosomal transforming acidic coiled-coil (TACC) protein Alp7 for spindle formation. Alp7 accumulates in the nucleus only during mitosis, although its underlying mechanism remains elusive. Here, we investigate the behaviour of Alp7 and its binding partner, Alp14/TOG, throughout the cell cycle. Interestingly, Alp7 enters the nucleus during interphase but is subsequently exported to the cytoplasm by the Exportin-dependent nuclear export machinery. The continuous nuclear export of Alp7 during interphase is essential for maintaining the array-like cytoplasmic microtubule structure. The mitosis-specific nuclear accumulation of Alp7 seems to be under the control of cyclin-dependent kinase (CDK). These results indicate that the spatiotemporal regulation of microtubule formation is established by the Alp7/TACC-Alp14/TOG complex through the coordinated interplay of Ran and CDK.

Citing Articles

Human Cytomegalovirus Exploits TACC3 To Control Microtubule Dynamics and Late Stages of Infection.

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TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells.

Furey C, Jovasevic V, Walsh D Cell Rep. 2020; 30(1):269-283.e6.

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Two XMAP215/TOG Microtubule Polymerases, Alp14 and Dis1, Play Non-Exchangeable, Distinct Roles in Microtubule Organisation in Fission Yeast.

Yukawa M, Kawakami T, Pinder C, Toda T Int J Mol Sci. 2019; 20(20).

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Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore.

Bao X, Spanos C, Kojidani T, Lynch E, Rappsilber J, Hiraoka Y Elife. 2018; 7.

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A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.

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