Apoptosis Caused by Hsp90 Inhibitor Geldanamycin in Leishmania Donovani During Promastigote-to-amastigote Transformation Stage

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2009 Aug 20

PMID 19690889

Citations 16

Authors

Qiaoli Li

Ying Zhou

Chenjiang Yao

Xiaoping Ma

Lianyun Wang

Wangjie Xu

Zhaoxia Wang

Zhongdong Qiao

Affiliations

Soon will be listed here.

Abstract

The role of heat shock protein 90 inhibitor geldanamycin (GA) during Leishmania donovani promastigote-to-amastigote transformation in axenic conditions was investigated. Promastigotes exhibited apoptotic morphologic changes after GA treatment at a high temperature, and the effect is in a dose- and time-dependant manner. Meanwhile, cell cycle analysis showed a significant increase at the expense of cells in the G0/G1 phase and a decrease in the S and G2/M phases after GA treatment. In addition, cellular glutathione level was reduced and reactive oxygen species content was increased afterwards. Pretreatment with antioxidants reduced the percentage of GA-induced cell apoptosis. After treatment, cultures in pH 5.5 showed a lower percentage of apoptosis than those in pH 7.4. The present study showed that GA could cause apoptosis in L. donovani but could not cause stage differentiation in high temperature and that acidic conditions were likely to be crucial for the transformation and survival of the parasite within its human host.

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