Chlamydia Trachomatis Alters Iron-regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells

Overview

Journal J Biomed Biotechnol

Specialty Biology

Date 2009 Aug 19

PMID 19688112

Citations 5

Authors

Harsh Vardhan

Apurb R Bhengraj

Rajneesh Jha

Aruna Singh Mittal

Affiliations

Soon will be listed here.

Abstract

Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in CT-infected HeLa-229 cells. Expression of iron-regulatory protein (IRP)-1 predominates over IRP-2 in infected cells. In infected cells, attenuated binding activity of IRP-iron responsive elements (IREs) is observed using the electrophoretic mobility-shift assay. These results suggest that iron homeostasis is modulated in CT-infected HeLa cells at the interface of acquisition and commensal use of iron.

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