Reprogramming Human Fibroblasts Using HIV-1 TAT Recombinant Proteins OCT4, SOX2, KLF4 and C-MYC

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2009 Aug 12

PMID 19669668

Citations 21

Authors

Chuanying Pan

Baisong Lu

Hong Chen

Colin E Bishop

Affiliations

Soon will be listed here.

Abstract

It has been shown that human and murine fibroblasts can be reprogrammed by ectopic expression of transcription factors using viral vectors. For the purpose of human therapeutic applications, the integration of viral transgenes into the genome is unlikely to be accepted. We therefore produced recombinant transcription factor proteins in E. coli (OCT4, SOX2, c-MYC and KLF4) carrying the cell penetrating TAT domain from HIV1. The purified proteins were able to enter into mammalian cells when added to tissue culture medium but appeared not to translocate to the nucleus. Further investigation indicated that most of the protein was tied up in the endosomes and was unavailable for reprogramming. Once this problem has been solved it seems likely that protein reprogramming will be the method of choice for clinical applications.

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