Thermal Characterization and Cytotoxicity of Complexes Formed by Papain and Cyclodextrin

Overview

Journal J Biol Phys

Specialty Biophysics

Date 2009 Aug 12

PMID 19669532

Citations 2

Authors

Gustavo H C Varca

Newton Andreo-Filho

Leonardo F Fraceto

Telma M Kaneko

Humberto G Ferraz

Natalia M Esteves

Michelle G Issa

Monica B Mathor

Patricia S Lopes

Affiliations

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Abstract

Papain is a proteolytic enzyme with restricted applications due to its limited stability. Cyclodextrins are widely used in pharmaceutical formulations once they are able to form complexes with other molecules, improving their stability and bioavailability. The purpose of the present paper was to analyze complexes formed by papain/hydroxypropyl-beta-cyclodextrin and papain/beta-cyclodextrin by thermal analysis and cytotoxicity tests to verify their possible interactions and toxicological behavior. Complex solutions were prepared at different molar ratios and collected as a function of time to be lyophilized and analyzed. Results showed changes in endothermic events and cytotoxicity profiles. A complex formation for both complexes is observed; nevertheless, beta-cyclodextrin was able to change the enzyme characteristics more efficiently.

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