Modulation of Glucocorticoid Receptor Induction Properties by Cofactors in Peripheral Blood Mononuclear Cells

Overview

Journal Hum Immunol

Specialty Allergy & Immunology

Date 2009 Aug 12

PMID 19665040

Citations 15

Authors

Min Luo

S Stoney Simons Jr

Affiliations

Soon will be listed here.

Abstract

Glucocorticoids are widely used for their anti-inflammatory and immunosuppressive properties. Changing concentrations of transcriptional cofactors or chemicals in transiently transfected tissue culture cells modify several properties of glucocorticoid receptor-regulated gene expression including total activity (A(max)), agonist steroid potency (EC(50)), and the percentage of full agonist activity for antisteroids (percent partial agonist activity). However, no reports exist for endogenous genes in primary human cells. Here we document that reduced concentrations of TIF2, a p160 coactivator, in peripheral blood mononuclear cells modulate these parameters for endogenous genes in a gene-selective manner, thus establishing the physiological relevance of this behavior.

Citing Articles

An Approach to Greater Specificity for Glucocorticoids.

Chow C, Simons Jr S Front Endocrinol (Lausanne). 2018; 9:76.

PMID: 29593646 PMC: 5859375. DOI: 10.3389/fendo.2018.00076.

Kinetically Defined Mechanisms and Positions of Action of Two New Modulators of Glucocorticoid Receptor-regulated Gene Induction.

Pradhan M, Blackford Jr J, Devaiah B, Thompson P, Chow C, Singer D J Biol Chem. 2015; 291(1):342-54.

PMID: 26504077 PMC: 4697169. DOI: 10.1074/jbc.M115.683722.

Theory of partial agonist activity of steroid hormones.

Chow C, Ong K, Kagan B, Simons Jr S AIMS Mol Sci. 2015; 2(2):101-123.

PMID: 25984562 PMC: 4430866. DOI: 10.3934/molsci.2015.2.101#sthash.jxRCteJz.dpuf.

Research resource: modulators of glucocorticoid receptor activity identified by a new high-throughput screening assay.

Blackford Jr J, Brimacombe K, Dougherty E, Pradhan M, Shen M, Li Z Mol Endocrinol. 2014; 28(7):1194-206.

PMID: 24850414 PMC: 4075162. DOI: 10.1210/me.2014-1069.

Minireview: dynamic structures of nuclear hormone receptors: new promises and challenges.

Simons Jr S, Edwards D, Kumar R Mol Endocrinol. 2013; 28(2):173-82.

PMID: 24284822 PMC: 3896641. DOI: 10.1210/me.2013-1334.

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