Molecular Immune Signatures of HIV-1 Vaccines in Human PBMCs

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2009 Aug 12

PMID 19665024

Citations 8

Authors

Alessandro Monaco

Francesco M Marincola

Marianna Sabatino

Zoltan Pos

Maria Lina Tornesello

David F Stroncek

Ena Wang

George K Lewis

Franco M Buonaguro

Luigi Buonaguro

Affiliations

Soon will be listed here.

Abstract

The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.

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References

Jin P, Wang E, Provenzano M, Deola S, Selleri S, Ren J . Molecular signatures induced by interleukin-2 on peripheral blood mononuclear cells and T cell subsets. J Transl Med. 2006; 4:26. PMC: 1557669. DOI: 10.1186/1479-5876-4-26. View

Wang E . RNA amplification for successful gene profiling analysis. J Transl Med. 2005; 3:28. PMC: 1201175. DOI: 10.1186/1479-5876-3-28. View

Borkow G, Bentwich Z . HIV and helminth co-infection: is deworming necessary?. Parasite Immunol. 2006; 28(11):605-12. DOI: 10.1111/j.1365-3024.2006.00918.x. View

Singh P, Kaur G, Sharma G, Mehra N . Immunogenetic basis of HIV-1 infection, transmission and disease progression. Vaccine. 2008; 26(24):2966-80. DOI: 10.1016/j.vaccine.2008.01.012. View

Xu X, Zhao Y, Simon R . Gene Set Expression Comparison kit for BRB-ArrayTools. Bioinformatics. 2007; 24(1):137-9. DOI: 10.1093/bioinformatics/btm541. View

Jin P, Zhao Y, Ngalame Y, Panelli M, Nagorsen D, Monsurro V . Selection and validation of endogenous reference genes using a high throughput approach. BMC Genomics. 2004; 5(1):55. PMC: 516027. DOI: 10.1186/1471-2164-5-55. View

Wang E, Miller L, Ohnmacht G, Mocellin S, Perez-Diez A, Petersen D . Prospective molecular profiling of melanoma metastases suggests classifiers of immune responsiveness. Cancer Res. 2002; 62(13):3581-6. PMC: 2241738. View

Nagorsen D, Deola S, Smith K, Wang E, Monsurro V, Zanovello P . Polarized monocyte response to cytokine stimulation. Genome Biol. 2005; 6(2):R15. PMC: 551535. DOI: 10.1186/gb-2005-6-2-r15. View

Shattock R, Haynes B, Pulendran B, Flores J, Esparza J . Improving defences at the portal of HIV entry: mucosal and innate immunity. PLoS Med. 2008; 5(4):e81. PMC: 2276525. DOI: 10.1371/journal.pmed.0050081. View

10.

Wang E, Miller L, Ohnmacht G, Liu E, Marincola F . High-fidelity mRNA amplification for gene profiling. Nat Biotechnol. 2000; 18(4):457-9. DOI: 10.1038/74546. View

11.

Buonaguro L, Tornesello M, Tagliamonte M, Gallo R, Wang L, Kamin-Lewis R . Baculovirus-derived human immunodeficiency virus type 1 virus-like particles activate dendritic cells and induce ex vivo T-cell responses. J Virol. 2006; 80(18):9134-43. PMC: 1563937. DOI: 10.1128/JVI.00050-06. View

12.

Arico E, Wang E, Tornesello M, Tagliamonte M, Lewis G, Marincola F . Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation. J Transl Med. 2005; 3:45. PMC: 1360684. DOI: 10.1186/1479-5876-3-45. View

13.

Doherty P, Turner S . The challenge of viral immunity. Immunity. 2007; 27(3):363-5. PMC: 7129985. DOI: 10.1016/j.immuni.2007.08.011. View

14.

Iqbal S, Kaul R . Mucosal innate immunity as a determinant of HIV susceptibility. Am J Reprod Immunol. 2007; 59(1):44-54. DOI: 10.1111/j.1600-0897.2007.00563.x. View

15.

Buonaguro L, Tornesello M, Gallo R, Marincola F, LEWIS G, Buonaguro F . Th2 polarization in peripheral blood mononuclear cells from human immunodeficiency virus (HIV)-infected subjects, as activated by HIV virus-like particles. J Virol. 2008; 83(1):304-13. PMC: 2612330. DOI: 10.1128/JVI.01606-08. View

16.

Lehner T, Wang Y, Pido-Lopez J, Whittall T, Bergmeier L, Babaahmady K . The emerging role of innate immunity in protection against HIV-1 infection. Vaccine. 2008; 26(24):2997-3001. DOI: 10.1016/j.vaccine.2007.11.060. View

17.

Buonaguro L, Monaco A, Arico E, Wang E, Tornesello M, Lewis G . Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation. BMC Bioinformatics. 2008; 9 Suppl 2:S5. PMC: 2323668. DOI: 10.1186/1471-2105-9-S2-S5. View

18.

Ross D, Scherf U, Eisen M, Perou C, Rees C, Spellman P . Systematic variation in gene expression patterns in human cancer cell lines. Nat Genet. 2000; 24(3):227-35. DOI: 10.1038/73432. View

19.

Buonaguro L, Buonaguro F, Tornesello M, Mantas D, Wagner R, Michelson S . High efficient production of Pr55(gag) virus-like particles expressing multiple HIV-1 epitopes, including a gp120 protein derived from an Ugandan HIV-1 isolate of subtype A. Antiviral Res. 2001; 49(1):35-47. DOI: 10.1016/s0166-3542(00)00136-4. View

20.

Buonaguro L, Visciano M, Tornesello M, Tagliamonte M, Biryahwaho B, Buonaguro F . Induction of systemic and mucosal cross-clade neutralizing antibodies in BALB/c mice immunized with human immunodeficiency virus type 1 clade A virus-like particles administered by different routes of inoculation. J Virol. 2005; 79(11):7059-67. PMC: 1112107. DOI: 10.1128/JVI.79.11.7059-7067.2005. View