The Mitochondrial Genome of an Exsymbiotic Chlorella-like Green Alga

Overview

Journal Plant Mol Biol

Date 1990 Feb 1

PMID 1966272

Citations 5

Authors

J A Waddle

A M Schuster

K W Lee

R H Meints

Affiliations

Soon will be listed here.

Abstract

Mitochondrial (mt) DNA from the unicellular, exsymbiotic Chlorella-like green alga, strain Nla was isolated and cloned. The mtDNA has a buoyant density of 1.692 g/ml in CsCl and an apparent G/C base composition of 32.5%. The genome contains approximately 76 kbp of DNA based on restriction fragment summation and electron microscopic measurements. A map of restriction endonuclease sites using Sst I, Bam I, Sal I and Xho I was generated. The genome maps as a circular molecule and appears as such under the electron microscope. Eight genes were assigned to the map by hybridization to specific restriction fragments using heterologous mt-encoded specific probes. These include the genes for subunits 6, 9, and alpha of the F0-F1 ATPase complex, the large and small subunit rRNAs, cytochrome oxidase subunits I and II, and apocytochrome b.

Citing Articles

Reaching for the ring: the study of mitochondrial genome structure.

Bendich A Curr Genet. 1993; 24(4):279-90.

PMID: 8252636 DOI: 10.1007/BF00336777.

The chloroplast genome of an exsymbiotic Chlorella-like green alga.

Schuster A, Waddle J, Korth K, Meints R Plant Mol Biol. 1990; 14(5):859-62.

PMID: 2102863 DOI: 10.1007/BF00016519.

Viruses and viruslike particles of eukaryotic algae.

Van Etten J, Lane L, Meints R Microbiol Rev. 1991; 55(4):586-620.

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Cloning and characterization of the Chlamydomonas moewusii mitochondrial genome.

Lee R, Dumas C, Lemieux C, Turmel M Mol Gen Genet. 1991; 231(1):53-8.

PMID: 1753945 DOI: 10.1007/BF00293821.

Large size and complex structure of mitochondrial DNA in two nonflowering land plants.

Palmer J, Soltis D, Soltis P Curr Genet. 1992; 21(2):125-9.

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