Molecular Diagnostic Tests for Microsporidia

Overview

Journal Interdiscip Perspect Infect Dis

Publisher Wiley

Date 2009 Aug 7

PMID 19657457

Citations 44

Authors

Kaya Ghosh

Louis M Weiss

Affiliations

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Abstract

The Microsporidia are a ubiquitous group of eukaryotic obligate intracellular parasites which were recognized over 100 years ago with the description of Nosema bombycis, a parasite of silkworms. It is now appreciated that these organisms are related to the Fungi. Microsporidia infect all major animal groups most often as gastrointestinal pathogens; however they have been reported from every tissue and organ, and their spores are common in environmental sources such as ditch water. Several different genera of these organisms infect humans, but the majority of infections are due to either Enterocytozoon bieneusi or Encephalitozoon species. These pathogens can be difficult to diagnose, but significant progress has been made in the last decade in the development of molecular diagnostic reagents for these organisms. This report reviews the molecular diagnostic tests that have been described for the identification of the microsporidia that infect humans.

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References

Orlandi P, Lampel K . Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa. J Clin Microbiol. 2000; 38(6):2271-7. PMC: 86779. DOI: 10.1128/JCM.38.6.2271-2277.2000. View

Wolk D, Schneider S, Wengenack N, Sloan L, Rosenblatt J . Real-time PCR method for detection of Encephalitozoon intestinalis from stool specimens. J Clin Microbiol. 2002; 40(11):3922-8. PMC: 139654. DOI: 10.1128/JCM.40.11.3922-3928.2002. View

Borchardt M, Spencer S . Concentration of Cryptosporidium, microsporidia and other water-borne pathogens by continuous separation channel centrifugation. J Appl Microbiol. 2002; 92(4):649-56. DOI: 10.1046/j.1365-2672.2002.01570.x. View

Coupe S, Delabre K, Pouillot R, Houdart S, Santillana-Hayat M, Derouin F . Detection of Cryptosporidium, Giardia and Enterocytozoon bieneusi in surface water, including recreational areas: a one-year prospective study. FEMS Immunol Med Microbiol. 2006; 47(3):351-9. DOI: 10.1111/j.1574-695X.2006.00098.x. View

Weiss L . Microsporidia: emerging pathogenic protists. Acta Trop. 2001; 78(2):89-102. DOI: 10.1016/s0001-706x(00)00178-9. View

Bart A, Wentink-Bonnema E, Heddema E, Buijs J, van Gool T . Frequent occurrence of human-associated microsporidia in fecal droppings of urban pigeons in amsterdam, the Netherlands. Appl Environ Microbiol. 2008; 74(22):7056-8. PMC: 2583493. DOI: 10.1128/AEM.01429-08. View

van Gool T, Vetter J, Weinmayr B, van Dam A, Derouin F, Dankert J . High seroprevalence of Encephalitozoon species in immunocompetent subjects. J Infect Dis. 1997; 175(4):1020-4. DOI: 10.1086/513963. View

Zhang Q, Singh I, Sheoran A, Feng X, Nunnari J, Carville A . Production and characterization of monoclonal antibodies against Enterocytozoon bieneusi purified from rhesus macaques. Infect Immun. 2005; 73(8):5166-72. PMC: 1201209. DOI: 10.1128/IAI.73.8.5166-5172.2005. View

Hylis M, Weiser J, Obornik M, Vavra J . DNA isolation from museum and type collection slides of microsporidia. J Invertebr Pathol. 2005; 88(3):257-60. DOI: 10.1016/j.jip.2005.02.004. View

10.

Dowd S, Gerba C, Pepper I . Confirmation of the human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in water. Appl Environ Microbiol. 1998; 64(9):3332-5. PMC: 106729. DOI: 10.1128/AEM.64.9.3332-3335.1998. View

11.

Ombrouck C, Ciceron L, Desportes-Livage I . Specific and rapid detection of Microsporidia in stool specimens from AIDS patients by PCR. Parasite. 1996; 3(1):85-6. DOI: 10.1051/parasite/1996031085. View

12.

Chan K, Koh T . Extraction of microsporidial DNA from modified trichrome-stained clinical slides and subsequent species identification using PCR sequencing. Parasitology. 2008; 135(6):701-3. DOI: 10.1017/S0031182008004411. View

13.

Visvesvara G . In vitro cultivation of microsporidia of clinical importance. Clin Microbiol Rev. 2002; 15(3):401-13. PMC: 118077. DOI: 10.1128/CMR.15.3.401-413.2002. View

14.

Visvesvara G, Leitch G, da Silva A, Croppo G, Moura H, Wallace S . Polyclonal and monoclonal antibody and PCR-amplified small-subunit rRNA identification of a microsporidian, Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection. J Clin Microbiol. 1994; 32(11):2760-8. PMC: 264156. DOI: 10.1128/jcm.32.11.2760-2768.1994. View

15.

David F, Schuitema A, Sarfati C, Liguory O, Hartskeerl R, Derouin F . Detection and species identification of intestinal microsporidia by polymerase chain reaction in duodenal biopsies from human immunodeficiency virus-infected patients. J Infect Dis. 1996; 174(4):874-7. DOI: 10.1093/infdis/174.4.874. View

16.

Notermans D, Peek R, de Jong M, Wentink-Bonnema E, Boom R, van Gool T . Detection and identification of Enterocytozoon bieneusi and Encephalitozoon species in stool and urine specimens by PCR and differential hybridization. J Clin Microbiol. 2005; 43(2):610-4. PMC: 548075. DOI: 10.1128/JCM.43.2.610-614.2005. View

17.

Vossbrinck C, Debrunner-Vossbrinck B . Molecular phylogeny of the Microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitol (Praha). 2005; 52(1-2):131-42. DOI: 10.14411/fp.2005.017. View

18.

Dong J, Olano J, McBride J, Walker D . Emerging pathogens: challenges and successes of molecular diagnostics. J Mol Diagn. 2008; 10(3):185-97. PMC: 2329782. DOI: 10.2353/jmoldx.2008.070063. View

19.

Graczyk T, Lucy F, Tamang L, Miraflor A . Human enteropathogen load in activated sewage sludge and corresponding sewage sludge end products. Appl Environ Microbiol. 2007; 73(6):2013-5. PMC: 1828822. DOI: 10.1128/AEM.02412-06. View

20.

Zhu X, Wittner M, Tanowitz H, Kotler D, Cali A, Weiss L . Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction. J Infect Dis. 1993; 168(6):1570-5. DOI: 10.1093/infdis/168.6.1570. View