Transcriptome Analysis of Taenia Solium Cysticerci Using Open Reading Frame ESTs (ORESTES)

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2009 Aug 4

PMID 19646239

Citations 15

Authors

Carolina R Almeida

Patricia H Stoco

Glauber Wagner

Thais Cm Sincero

Gianinna Rotava

Ethel Bayer-Santos

Juliana B Rodrigues

Maisa M Sperandio

Antonio Am Maia

Elida Pb Ojopi

Arnaldo Zaha

Henrique B Ferreira

Kevin M Tyler

Alberto Mr Davila

Edmundo C Grisard

Emmanuel Dias-Neto

Affiliations

Soon will be listed here.

Abstract

Background: Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form.

Results: Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development.

Conclusion: The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.

Citing Articles

Proteomic analysis of cysticercus and adult stages.

Li L, He W, Fan X, Liu M, Luo B, Yang F Front Vet Sci. 2023; 9:934197.

PMID: 36699330 PMC: 9868161. DOI: 10.3389/fvets.2022.934197.

Taenia solium Cysticercosis and Its Impact in Neurological Disease.

Garcia H, Gonzalez A, Gilman R Clin Microbiol Rev. 2020; 33(3).

PMID: 32461308 PMC: 7254859. DOI: 10.1128/CMR.00085-19.

A gel-free proteomic analysis of Taenia solium and Taenia crassiceps cysticerci vesicular extracts.

Verissimo da Costa G, Peralta R, Kalume D, Alves A, Peralta J Parasitol Res. 2018; 117(12):3781-3790.

PMID: 30215138 DOI: 10.1007/s00436-018-6080-7.

A Role for Epitope Networking in Immunomodulation by Helminths.

Homan E, Bremel R Front Immunol. 2018; 9:1763.

PMID: 30108588 PMC: 6079203. DOI: 10.3389/fimmu.2018.01763.

Molecular Cloning of a cDNA Encoding for Taenia solium TATA-Box Binding Protein 1 (TsTBP1) and Study of Its Interactions with the TATA-Box of Actin 5 and Typical 2-Cys Peroxiredoxin Genes.

Rodriguez-Lima O, Garcia-Gutierrez P, Jimenez L, Zarain-Herzberg A, Lazzarini R, Landa A PLoS One. 2015; 10(11):e0141818.

PMID: 26529408 PMC: 4631506. DOI: 10.1371/journal.pone.0141818.

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