Molecular and Biochemical Characterization of AtPAP15, a Purple Acid Phosphatase with Phytase Activity, in Arabidopsis

Overview

Journal Plant Physiol

Specialty Physiology

Date 2009 Jul 28

PMID 19633233

Citations 41

Authors

Ruibin Kuang

Kam-Ho Chan

Edward Yeung

Boon Leong Lim

Affiliations

Soon will be listed here.

Abstract

Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with soybean (Glycine max; GmPHY) and tobacco (Nicotiana tabacum) PAP (NtPAP) with phytase activity than the other AtPAPs. In this study, transgenic Arabidopsis that expressed an AtPAP15 promoterbeta-glucuronidase (GUS) fusion protein showed that AtPAP15 expression was developmentally and temporally regulated, with strong GUS staining at the early stages of seedling growth and pollen germination. The expression was also organ/tissue specific, with strongest GUS staining in the vasculature, pollen grains, and roots. The recombinant AtPAP purified from transgenic tobacco exhibited broad substrate specificity with moderate phytase activity. AtPAP15 T-DNA insertion lines exhibited a lower phytase and phosphatase activity in seedling and germinating pollen and lower pollen germination rate compared with the wild type and their complementation lines. Therefore, AtPAP15 likely mobilizes phosphorus reserves in plants, particularly during seed and pollen germination. Since AtPAP15 is not expressed in the root hair or in the epidermal cells, it is unlikely to play any role in external phosphorus assimilation.

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References

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Bendtsen J, Nielsen H, von Heijne G, Brunak S . Improved prediction of signal peptides: SignalP 3.0. J Mol Biol. 2004; 340(4):783-95. DOI: 10.1016/j.jmb.2004.05.028. View

Veljanovski V, Vanderbeld B, Knowles V, Snedden W, Plaxton W . Biochemical and molecular characterization of AtPAP26, a vacuolar purple acid phosphatase up-regulated in phosphate-deprived Arabidopsis suspension cells and seedlings. Plant Physiol. 2006; 142(3):1282-93. PMC: 1630754. DOI: 10.1104/pp.106.087171. View

Bozzo G, Raghothama K, Plaxton W . Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures. Biochem J. 2003; 377(Pt 2):419-28. PMC: 1223867. DOI: 10.1042/BJ20030947. View

Richardson A, Hadobas P, Hayes J . Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate. Plant J. 2001; 25(6):641-9. DOI: 10.1046/j.1365-313x.2001.00998.x. View

Cashikar A, Kumaresan R, Rao N . Biochemical Characterization and Subcellular Localization of the Red Kidney Bean Purple Acid Phosphatase. Plant Physiol. 1997; 114(3):907-915. PMC: 158379. DOI: 10.1104/pp.114.3.907. View

Vogel A, Borchers T, Marcus K, Meyer H, Krebs B, Spener F . Heterologous expression and characterization of recombinant purple acid phosphatase from red kidney bean. Arch Biochem Biophys. 2002; 401(2):164-72. DOI: 10.1016/S0003-9861(02)00046-2. View

Valpuesta V, Botella M . Biosynthesis of L-ascorbic acid in plants: new pathways for an old antioxidant. Trends Plant Sci. 2004; 9(12):573-7. DOI: 10.1016/j.tplants.2004.10.002. View

Zhu H, Qian W, Lu X, Li D, Liu X, Liu K . Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower. Plant Mol Biol. 2005; 59(4):581-94. DOI: 10.1007/s11103-005-0183-0. View

10.

Del Pozo J, Allona I, Rubio V, Leyva A, de la Pena A, Aragoncillo C . A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions. Plant J. 1999; 19(5):579-89. DOI: 10.1046/j.1365-313x.1999.00562.x. View

11.

Ibrahim H, Pertl H, Pittertschatscher K, Bentrup F, Obermeyer G . Release of an acid phosphatase activity during lily pollen tube growth involves components of the secretory pathway. Protoplasma. 2002; 219(3-4):176-83. DOI: 10.1007/s007090200019. View

12.

Li D, Zhu H, Liu K, Liu X, Leggewie G, Udvardi M . Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation. J Biol Chem. 2002; 277(31):27772-81. DOI: 10.1074/jbc.M204183200. View

13.

Bassuner B, Lam R, Lukowitz W, Yeung E . Auxin and root initiation in somatic embryos of Arabidopsis. Plant Cell Rep. 2006; 26(1):1-11. DOI: 10.1007/s00299-006-0207-5. View

14.

Fan L, Wang Y, Wang H, Wu W . In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts. J Exp Bot. 2001; 52(361):1603-14. View

15.

Flanagan J, Cassady A, Schenk G, Guddat L, Hume D . Identification and molecular modeling of a novel, plant-like, human purple acid phosphatase. Gene. 2006; 377:12-20. DOI: 10.1016/j.gene.2006.02.031. View

16.

Schenk G, Ge Y, Carrington L, Wynne C, Searle I, Carroll B . Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean. Arch Biochem Biophys. 1999; 370(2):183-9. DOI: 10.1006/abbi.1999.1407. View

17.

Hegeman C, Grabau E . A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings. Plant Physiol. 2001; 126(4):1598-608. PMC: 117159. DOI: 10.1104/pp.126.4.1598. View

18.

Horsch R, Rogers S, Fraley R . Transgenic plants. Cold Spring Harb Symp Quant Biol. 1985; 50:433-7. DOI: 10.1101/sqb.1985.050.01.054. View

19.

Bozzo G, Raghothama K, Plaxton W . Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures. Eur J Biochem. 2002; 269(24):6278-86. DOI: 10.1046/j.1432-1033.2002.03347.x. View

20.

Zhang W, Gruszewski H, Chevone B, Nessler C . An Arabidopsis purple acid phosphatase with phytase activity increases foliar ascorbate. Plant Physiol. 2007; 146(2):431-40. PMC: 2245855. DOI: 10.1104/pp.107.109934. View