Escherichia Coli DksA Binds to Free RNA Polymerase with Higher Affinity Than to RNA Polymerase in an Open Complex

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2009 Jul 21

PMID 19617357

Citations 24

Authors

Christopher W Lennon

Tamas Gaal

Wilma Ross

Richard L Gourse

Affiliations

Soon will be listed here.

Abstract

The transcription factor DksA binds in the secondary channel of RNA polymerase (RNAP) and alters transcriptional output without interacting with DNA. Here we present a quantitative assay for measuring DksA binding affinity and illustrate its utility by determining the relative affinities of DksA for three different forms of RNAP. Whereas the apparent affinities of DksA for RNAP core and holoenzyme are the same, the apparent affinity of DksA for RNAP decreases almost 10-fold in an open complex. These results suggest that the conformation of RNAP present in an open complex is not optimal for DksA binding and that DNA directly or indirectly alters the interface between the two proteins.

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