Promotion of Stem Cell Proliferation by Vegetable Peptone

Overview

Journal Cell Prolif

Date 2009 Jul 21

PMID 19614679

Citations 2

Authors

J Lee

H Hwang

E Jung

S Huh

J Hyun

D Park

Affiliations

Soon will be listed here.

Abstract

Objectives: Technical limitations and evolution of therapeutic applications for cell culture-derived products have accelerated elimination of animal-derived constituents from such products to minimize inadvertent introduction of microbial contaminants, such as fungi, bacteria or viruses. The study described here was conducted to investigate the proliferative effect of vegetable peptone on adult stem cells in the absence of serum, and its possible mechanisms of action.

Materials And Methods: Cell viability and proliferation were determined using the MTT assay and Click-iT EdU flow cytometry, respectively. In addition, changes in expression of cytokine genes were analysed using MILLIPLEX human cytokine enzyme-linked immunosorbent assay kit.

Results: Viability of cord blood-derived mesenchymal stem cells (CB-MSC) and adipose tissue-derived stem cells (ADSC) increased significantly when treated with the peptone. In addition, median value of the group treated with peptone shifted to the right when compared to the untreated control group. Furthermore, quantitative analysis of the cytokines revealed that production of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-beta1), and interleukin-6 (IL-6) increased significantly in response to treatment with our vegetable peptone in both CB-MSCs and ADSCs.

Conclusions: Our findings revealed that the vegetable peptone promotes proliferation of CB-MSCs and ADSCs. In addition, results of this study suggest that induction of stem cell proliferation by vegetable peptone is likely to be related to its induction of VEGF, TGF-beta1, and IL-6 expression.

Citing Articles

Systemic Factors During Metabolic Disease Progression Contribute to the Functional Decline of Adipose Tissue-Derived Mesenchymal Stem Cells in Reproductive Aged Females.

Seboko A, Conradie M, Kruger M, Ferris W, Conradie M, van de Vyver M Front Physiol. 2019; 9:1812.

PMID: 30631282 PMC: 6315119. DOI: 10.3389/fphys.2018.01812.

Adipose-derived stem cells for clinical applications: a review.

Wilson A, Butler P, Seifalian A Cell Prolif. 2011; 44(1):86-98.

PMID: 21199013 PMC: 6496460. DOI: 10.1111/j.1365-2184.2010.00736.x.

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